General information:
Bovine Cervical mucin
Growth/harvest conditions for recombinantly produced material:
NA
Treatments and/or storage conditions:
Mucins isolated by isopycnic centrifugation
For commercial material, vendor and applicable item information:
NA
Generation of chemically derived material:
NA
Purification steps:
After reductive b elimination, salts are removed by cation exchange beads packed in zip tip pipets, eluted with water, samples were lyphilized and borate complexes removed by repeated MeOH addition/evaporation
Sample name:
O-linked oligosaccharides
Instrument: Agilent 1100 Series
Manufacturer url: https://www.agilent.com/home
Injector: PAL Systems. HTC PAL
Injector settings: 3 ul drawn injected into a 2 ul loop
Manufacturer: In-house
Model: NA
Type of Chromatography: Graphitized carbon
Type of Material: Graphitized carbon
Column diameter: 250 um
Column length: 10 cm
Particle size: 10 u
Manufacturer url: NA
Temperature: RT
Solvent a: 10 mM (NH4)HCO3
Solvent b: 10 mM (NH4)HCO3 in 80% acetonitrile
Other solvent: NA
Flow rate: Approx 3-5 ul/min (static split from 250 ul/min)
Flow gradient: 0-50% B in 40 min, 50-100% B from 40-48 min
Run time: 78 min
Phase: NA
Date stamp: Thu Sep 14 UTC 2017
Instrument: Thermo Fisher LTQ Linear Ion Trap ESI
Customizations from the manufacturer's specification:
None
ion_mode: -
Sprayer type : Liquid chromatography
Interface name: NA
Other data for the Interface: Thermo Fisher Electrospray
Sprayer name: Electrospray
Other data for the sprayer: Thermo Fisher metal needle
Relevant voltages: Source Voltage 2.5 kV, Capillary Voltage -15 V
Degree of prompt fragmentation evaluated: Yes, no sign of prompt fragmentation as found in supplied BCM259-1.raw data file
In-source dissociation performed: No
Other parameters if discriminant for the experiment: NA
Plate composition (or type): NA
Matrix composition: NA
Deposition technique: NA
Relevant voltages: NA
Degree of prompt fragmentation evaluated: NA
PSD (or LID/ISD) summary: NA
Operation with or without delayed extraction: NA
Laser type: NA
Other laser related parameters: NA
url: NA
Hardware options: NA
CID. Gas composition: He
CID. Gas pressure: NA
CID. Collision energy: Normalised energy 35
ETD. Reagent gas: NA
ETD. Pressure: NA
ETD. Reaction time: NA
ETD. Reagent atoms: NA
ECD. Emitter type: NA
ECD. Voltage: NA
ECD. Current: NA
TOF. Reflectron status: NA
Ion trap. Final MS stage achieved: MS2
Ion mobility. Gas type : NA
Ion mobility. Pressure: NA
Ion mobility. Instrument parameters: NA
Peak selection: NA
FT-ICR. Pulse: NA
FT-ICR. Width: NA
FT-ICR. IR: Decay time: NA
FT-ICR. IR: NA
FT-ICR. Other parameters: NA
Detector type: Electron Multiplier