The Glycomic MS Database and Repository

Responsible:

Rebecca Miller . University Of Copenhagen . rmiller@sund.ku.dk

Sample preparation




HPLC


1. General settings

Instrument: NA

Manufacturer url: NA

Injector: NA

Injector settings: NA


2. Column

Manufacturer:

Model:

Type of Chromatography:

Type of Material:

Column diameter:

Column length:

Particle size:

Manufacturer url: NA


3. Method run

Temperature:

Solvent a: water

Solvent b: water

Other solvent:

Flow rate: 0.1 ml/min

Flow gradient:

Run time:

Phase: none



MS



1. General features

Date stamp: Tue Feb 18 00:00:00 UTC 2020

Instrument: Waters Synapt G2-S HDMS

Customizations from the manufacturer's specification:
DTIMS (Triwave removed)

ion_mode: -


2. Ion sources

(a) Electrospray ionisation - ESI

Sprayer type : static

Interface name:

Other data for the Interface:

Sprayer name: NanoLock Spray Ionisation Source

Other data for the sprayer: Pd/Pt-coated borosilicate nanoelectrospray ionization (nESI) capillaries

Relevant voltages: Capillary Voltage: 0.65kV, Cone Voltage 2, Source Temp 100oC,

Degree of prompt fragmentation evaluated: Not applicable

In-source dissociation performed: No

Other parameters if discriminant for the experiment:


(b) MALDI

Plate composition (or type): NA

Matrix composition: NA

Deposition technique: NA

Relevant voltages: NA

Degree of prompt fragmentation evaluated: NA

PSD (or LID/ISD) summary: NA

Operation with or without delayed extraction: NA

Laser type: NA

Other laser related parameters: NA

url: NA


3. Ion transfer optics

Hardware options:


3. Post-source componentry

Collision-Induced Dissociation - CID

CID. Gas composition: Argon

CID. Gas pressure:

CID. Collision energy: For MS/MS experiments the trap collision energy was increased to 3V–18 V.

Electron Transfer Dissociation - ETD

ETD. Reagent gas: NA

ETD. Pressure: NA

ETD. Reaction time: NA

ETD. Reagent atoms: NA

Electron Capture Dissociation - ECD

ECD. Emitter type: NA

ECD. Voltage: NA

ECD. Current: NA

TOF drift tube

TOF. Reflectron status: NA

Ion Trap

Ion trap. Final MS stage achieved: NA

Ion mobility

Ion mobility. Gas type : Helium

Ion mobility. Pressure:

Ion mobility. Instrument parameters: Trap gas flow, 2 ml/min; helium cell gas flow, 180 ml/min; ion mobility gas flow, 20 ml/min; trap bias, 2V; ion mobility wave velocity, 300 m/s; ion mobility wave height, 0 V.

FT-ICR

Peak selection: NA

FT-ICR. Pulse: NA

FT-ICR. Width: NA

FT-ICR. IR: Decay time: NA

FT-ICR. IR: NA

FT-ICR. Other parameters: NA

Detectors

Detector type: NA