A complex connection between the diversity of human gastric mucin O-glycans, Helicobacter pylori binding, Helicobacter infection and fucosylation

Responsible:

Chunsheng Jin . Gothenburg University . chunsheng.jin@medkem.gu.se

Sample preparation

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1.Sample Origin

General information:
Human gastric mucins from patients with cancer. The mucins were isolated either from healthy or diseased tissue.

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
NA

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
NA

2. Defined Sample

Sample name:
NA

HPLC

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1. General settings

Instrument: NA

Manufacturer url: NA

Injector: NA

Injector settings: NA

2. Column

Manufacturer: NA

Model: NA

Type of Chromatography: NA

Type of Material: NA

Column diameter: NA

Column length: NA

Particle size: NA

Manufacturer url: NA

3. Method run

Temperature: NA

Solvent a: 10 mM NH4HCO3

Solvent b: 10 mM NH4HCO3 in 80% AcCN

Other solvent: NA

Flow rate: 10-20 ul/min

Flow gradient: NA

Run time: NA

Phase: Reverse phase

MS

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1. General features

Date stamp: Mon Oct 01 UTC 2018

Instrument: Agilent 6310 Ion Trap LC/MS

Customizations from the manufacturer's specification:
NA

ion_mode: -

2. Ion sources

(a) Electrospray ionisation - ESI

Sprayer type : fed

Interface name: NA

Other data for the Interface: NA

Sprayer name: API

Other data for the sprayer: NA

Relevant voltages: NA

Degree of prompt fragmentation evaluated: Yes

In-source dissociation performed: Yes

Other parameters if discriminant for the experiment: NA

(b) MALDI

Plate composition (or type): NA

Matrix composition: NA

Deposition technique: NA

Relevant voltages: NA

Degree of prompt fragmentation evaluated: NA

PSD (or LID/ISD) summary: NA

Operation with or without delayed extraction: NA

Laser type: NA

Other laser related parameters: NA

url: https://www.agilent.com/home

3. Ion transfer optics

Hardware options: NA

3. Post-source componentry

Collision-Induced Dissociation - CID

CID. Gas composition: air

CID. Gas pressure: NA

CID. Collision energy: 35%

Electron Transfer Dissociation - ETD

ETD. Reagent gas: NA

ETD. Pressure: NA

ETD. Reaction time: NA

ETD. Reagent atoms: NA

Electron Capture Dissociation - ECD

ECD. Emitter type: NA

ECD. Voltage: NA

ECD. Current: NA

TOF drift tube

TOF. Reflectron status: NA

Ion Trap

Ion trap. Final MS stage achieved: NA

Ion mobility

Ion mobility. Gas type : NA

Ion mobility. Pressure: NA

Ion mobility. Instrument parameters: NA

FT-ICR

Peak selection: NA

FT-ICR. Pulse: NA

FT-ICR. Width: NA

FT-ICR. IR: Decay time: NA

FT-ICR. IR: NA

FT-ICR. Other parameters: NA

Detectors

Detector type: NA