General information:
-
Growth/harvest conditions for recombinantly produced material:
-
Treatments and/or storage conditions:
-
For commercial material, vendor and applicable item information:
-
Generation of chemically derived material:
-
Purification steps:
-
Sample name:
O-linked oligosaccharides
Instrument: Thermo Fisher 1100 Series
Manufacturer url: NA
Injector: NA
Injector settings: NA
Manufacturer: In-house
Model: NA
Type of Chromatography: NA
Type of Material: Porous graphitized carbon
Column diameter: 0.25
Column length: 100
Particle size: 5
Manufacturer url: NA
Temperature: RT
Solvent a: 10 mM (NH4)HCO3 in water
Solvent b: 10 mM (NH4)HCO3 in 80% acetontirile
Other solvent: NA
Flow rate: 10 ul/min
Flow gradient: 0-40%
Run time: 40 min
Phase: Reverse phase
Date stamp: Tue Oct 19 UTC 2021
Instrument: Thermo Fisher LTQ Linear Ion Trap ESI
Customizations from the manufacturer's specification:
NA
ion_mode: -
Sprayer type : Fed
Interface name: NA
Other data for the Interface: NA
Sprayer name: IonMax standard ESI source
Other data for the sprayer: Equipped with a stainless steel needle
Relevant voltages: Electrospray voltage of 3.5 kV and capillary voltage of 33.0 V.
Degree of prompt fragmentation evaluated: Yes
In-source dissociation performed: Yes
Other parameters if discriminant for the experiment: NA
Plate composition (or type): NA
Matrix composition: NA
Deposition technique: NA
Relevant voltages: NA
Degree of prompt fragmentation evaluated: NA
PSD (or LID/ISD) summary: NA
Operation with or without delayed extraction: NA
Laser type: NA
Other laser related parameters: NA
url: NA
Hardware options: NA
CID. Gas composition: He
CID. Gas pressure: NA
CID. Collision energy: Collision energy 35%
ETD. Reagent gas: NA
ETD. Pressure: NA
ETD. Reaction time: NA
ETD. Reagent atoms: NA
ECD. Emitter type: NA
ECD. Voltage: NA
ECD. Current: NA
TOF. Reflectron status: NA
Ion trap. Final MS stage achieved: NA
Ion mobility. Gas type : NA
Ion mobility. Pressure: NA
Ion mobility. Instrument parameters: NA
Peak selection: NA
FT-ICR. Pulse: NA
FT-ICR. Width: NA
FT-ICR. IR: Decay time: NA
FT-ICR. IR: NA
FT-ICR. Other parameters: NA
Detector type: NA