Human FSH Glycoform a-Subunit Asparagine52 Glycans: Major 1 Glycan Structural 2 Consistency, Minor Glycan Variation in Abundance

Responsible:

George R. Bousfield . Wichita State University . george.bousfield@wichita.edu

Sample preparation

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1.Sample Origin

General information:
Human pituitary glands and pituitary hFSH were obtained from the National Hormone and Pituitary Program

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
National Hormone and Pituitary Program

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
Nafion ion exchange membrane

2. Defined Sample

Sample name:
N-linked oligosaccharides

1.Sample Origin

General information:
Human pituitary glands and pituitary hFSH were obtained from the National Hormone and Pituitary Program

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
National Hormone and Pituitary Program

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
Nafion ion exchange membrane

2. Defined Sample

Sample name:
N-linked oligosaccharides

1.Sample Origin

General information:
Human pituitary glands and pituitary hFSH were obtained from the National Hormone and Pituitary Program

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
National Hormone and Pituitary Program

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
Nafion ion exchange membrane

2. Defined Sample

Sample name:
N-linked oligosaccharides

1.Sample Origin

General information:
Human pituitary glands and pituitary hFSH were obtained from the National Hormone and Pituitary Program

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
National Hormone and Pituitary Program

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
Nafion ion exchange membrane

2. Defined Sample

Sample name:
N-linked oligosaccharides

HPLC

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N/A

MS

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1. General features

Date stamp: Fri Mar 01 UTC 1907

Instrument: Waters Q-Tof Ultima

Customizations from the manufacturer's specification:
NA

ion_mode: +

2. Ion sources

(a) Electrospray ionisation - ESI

Sprayer type : Static

Interface name: Proxeon nanospray emitter

Other data for the Interface: Proxeon

Sprayer name: Nanospray

Other data for the sprayer: NA

Relevant voltages: Tip = 1.2 kV

Degree of prompt fragmentation evaluated: Not applicable

In-source dissociation performed: No

Other parameters if discriminant for the experiment: NA

(b) MALDI

Plate composition (or type): NA

Matrix composition: NA

Deposition technique: NA

Relevant voltages: NA

Degree of prompt fragmentation evaluated: NA

PSD (or LID/ISD) summary: NA

Operation with or without delayed extraction: NA

Laser type: NA

Other laser related parameters: NA

url: NA

3. Ion transfer optics

Hardware options: Q-TOF

3. Post-source componentry

Collision-Induced Dissociation - CID

CID. Gas composition: Argon

CID. Gas pressure: NA

CID. Collision energy: Variable

Electron Transfer Dissociation - ETD

ETD. Reagent gas: NA

ETD. Pressure: NA

ETD. Reaction time: NA

ETD. Reagent atoms: NA

Electron Capture Dissociation - ECD

ECD. Emitter type: NA

ECD. Voltage: NA

ECD. Current: NA

TOF drift tube

TOF. Reflectron status: On

Ion Trap

Ion trap. Final MS stage achieved: NA

Ion mobility

Ion mobility. Gas type : NA

Ion mobility. Pressure: NA

Ion mobility. Instrument parameters: NA

FT-ICR

Peak selection: NA

FT-ICR. Pulse: NA

FT-ICR. Width: NA

FT-ICR. IR: Decay time: NA

FT-ICR. IR: NA

FT-ICR. Other parameters: NA

Detectors

Detector type: NA