Mucin Producing In vitro Fish Mucosal Surfaces as Models for Studying Host-Pathogen Interactions

Responsible:

Kristina Thomsson . Institute of Biomedicine, University of Gothenburg . kristina.thomsson@medkem.gu.se

Sample preparation

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1.Sample Origin

General information:
Glycoproteins from embryonic Chinook salmon cell line (CHSE-214) and rainbow trout gill cell line (RTGgillW1)

Growth/harvest conditions for recombinantly produced material:
The cells were stimulated into mucus-producing cells using DAPT and IWP-2. Glycoproteins were extracted using guanidinium hydrochloride.

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
NA

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
After reductive b elimination, salts are removed by cation exchange beads packed in zip tip pipets, eluted with water, samples were lyphilized and borate complexes removed by repeated MeOH addition/evaporation

2. Defined Sample

Sample name:
O-linked oligosaccharides

HPLC

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N/A

MS

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N/A