Glycan complexity and heterogeneity of glycosylation proteins of the infective stage of the helminth Fasciola hepatica

Responsible:

Chunsheng Jin . Institute of Biomedicine, Gothenburg University . chunsheng.jin@medkem.gu.se

Sample preparation

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1.Sample Origin

General information:
Membrane protein samples from zebrafish (Danio rerio) heart

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
NA

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
NA

2. Defined Sample

Sample name:
NA

HPLC

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1. General settings

Instrument: Agilent 1100 Series

Manufacturer url: https://www.agilent.com/home

Injector: NA

Injector settings: NA

2. Column

Manufacturer: NA

Model: NA

Type of Chromatography: NA

Type of Material: NA

Column diameter: NA

Column length: NA

Particle size: NA

Manufacturer url: NA

3. Method run

Temperature: NA

Solvent a: 10 mM Ammonium Biocarbonate

Solvent b: 10 mM Ammonium Biocarbonate in 80% AcCN

Other solvent: NA

Flow rate: 10 ul/min

Flow gradient: NA

Run time: NA

Phase: normal phase

MS

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N/A