General information:
Proteins from unfiltered BAL lysates from healthy individuals, long term smokers and COPD patients
Growth/harvest conditions for recombinantly produced material:
NA
Treatments and/or storage conditions:
NA
For commercial material, vendor and applicable item information:
NA
Generation of chemically derived material:
NA
Purification steps:
NA
Sample name:
N-linked oligosaccharides
Instrument: Agilent 1100 Series
Manufacturer url: https://www.agilent.com/home
Injector: Pal systems - HTC Pal
Injector settings: 3 ul drawn injected into a 2 ul loop
Manufacturer: In-house
Model: NA
Type of Chromatography: Graphitized carbon
Type of Material: Graphitized carbon
Column diameter: 250 um
Column length: 10 cm
Particle size: 10 u
Manufacturer url: NA
Temperature: RT
Solvent a: 10 mM (NH4)HCO3
Solvent b: 10 mM (NH4)HCO3 in 80% acetonitrile
Other solvent: NA
Flow rate: approx 3-5 ul/min (static split from 250 ul/min)
Flow gradient: 0-50% B in 40 min, 50-100% B from 40-48 min
Run time: 78 min
Phase: Reversed phase
N/A