Ion Mobility-Tandem Mass Spectrometry of Mucin-type O-Glycans: from Porcine gastric mucins

Responsible:

Bechtella, Leïla . Freie Universität Berlin . leila.bechtella@fu-berlin.de

Sample preparation

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1.Sample Origin

General information:
mucin-type O-glycans

Growth/harvest conditions for recombinantly produced material:
NA

Treatments and/or storage conditions:
NA

For commercial material, vendor and applicable item information:
Sigma-Aldrich, Mucin from porcine stomach (type III)

Generation of chemically derived material:
NA

2. Sample Processing

Purification steps:
desalting on cation exchange beads, enrichment on PGC SPE cartridge

2. Defined Sample

Sample name:
O-linked oligosaccharides

HPLC

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N/A

MS

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N/A