MIRAGE upload

redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd

Recombinant mucin-type proteins carrying LacdiNAc on different O-glycan core chains fail to support H. pylori binding

GlyTouCan Accession Number: Loading...

Peak	Intensity
178.05	0.6
178.99	1.7
183.93	2.8
184.55	1.2
185.74	0.4
195.82	1.2
201.95	64.8
202.74	2.4
203.09	3.3
203.96	37.2
204.87	1.2
205.13	1.0
207.86	0.5
209.08	0.8
214.82	0.4
217.8	2.0
218.85	1.0
219.81	80.2
220.68	7.7
221.03	4.0
221.39	0.9
221.98	48.0
223.01	2.4
224.95	0.5
225.93	0.6
239.09	0.9
240.14	1.0
243.77	0.6
257.99	0.5
261.97	672.1
263.0	65.4
270.12	0.4
272.91	0.9
275.96	6.4
279.43	0.4
284.89	0.5
299.05	4.6
300.24	0.6
303.93	392.0
304.93	42.7
316.86	1.9
321.85	210.9
322.89	21.5
327.09	0.6
339.27	0.6
344.71	8.0
345.19	3.8
346.02	1.5
346.94	1.9
348.11	1.0
359.05	2.6
362.96	0.4
368.86	1.1
370.07	0.6
376.72	0.4
380.76	0.4
383.52	0.6
386.97	1.5
389.03	1.6
405.04	131.9
405.99	24.7
407.07	132.4
408.03	19.6
409.59	0.3
423.02	5.2
424.11	1.1
425.02	29.8
425.85	1.4
426.4	2.6
478.16	3.0
479.31	2.5
502.3	2.3
520.03	76.6
520.87	7.6
521.24	5.4
524.88	0.9
532.34	2.7
537.89	1.0
538.53	0.3
549.98	0.5
554.83	1.3
561.66	0.6
562.16	3.0
563.13	1.3
566.64	1.4
568.1	64.5
569.2	14.1
580.97	1.2
581.54	0.9
586.07	39.3
587.07	9.1
591.79	0.9
592.65	1.0
593.77	0.5
602.53	0.4
609.84	6.5
610.36	1.8
610.91	2.7
611.92	1.0
629.55	0.4

There are 3 similar spectra:

Id	Structure	Score
5549		0.95
3870		0.79
3744		0.66

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 627.26
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column Hypercarb

Method

Over 40 min gradient with 0.010 mL/min run rate
10 mM NH4HCO3
0-40% acetonitrile in 10 mM NH4HCO3

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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