Structure

    freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S)--6?1S$MONO,Und,-2H,0,freeEnd

Shotgun Ion-Mobility Mass Spectrometry Sequencing (SIMMS2) for glycosaminoglycan oligosaccharides

tag attached

GlyTouCan Accession Number: Loading...

Peak Intensity
85.0414 494.0
113.0402 2070.0

Annotations:

^{2,5}A_{GlcNAc}Z b1D-GlcNAc,p/#acleavage_2_5(--4a1D-GlcA,p--4b1D-GlcNAc,p/#zcleavage)--6?1S$MONO,Und,-3H,0,freeEnd
237.5667 642.0

Annotations:

C a1D-GlcA,p/#ccleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-2H,0,freeEnd CY b1D-GlcNAc,p/#ccleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-2H,0,freeEnd Y freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p/#ycleavage)--6?1S$MONO,Und,-2H,0,freeEnd
268.5442 260.0
277.0969 1910.0

Annotations:

^{0,2}A_{GlcNAc}Z b1D-GlcNAc,p/#acleavage_0_2(--4a1D-GlcA,p--4b1D-GlcNAc,p/#zcleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd ^{0,2}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_0_2(--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S)--6?1S$MONO,Und,-3H,0,freeEnd
280.1187 292.0
282.0717 2920.0

Annotations:

BY a1D-GlcA,p/#bcleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd CZ a1D-GlcA,p/#ccleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd Z freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd
283.0724 259.0
284.0717 155.0
300.0829 660.0

Annotations:

CY a1D-GlcA,p/#ccleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd Y freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd
305.2195 157.0
316.5911 1060.0

Annotations:

B b1D-GlcNAc,p/#bcleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-2H,0,freeEnd
316.8529 21.7
378.1615 1300.0

Annotations:

CZ b1D-GlcNAc,p/#ccleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd Z freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p/#zcleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd B a1D-GlcA,p/#bcleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd BY b1D-GlcNAc,p/#bcleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd
380.6696 79.8
381.1627 108.0
381.6764 5310.0
381.7777 34.5

Annotations:

^{2,5}A_{GlcA}Z a1D-GlcA,p/#acleavage_2_5--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd ^{1,4}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_1_4--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-2H,0,freeEnd
382.1771 1850.0

Annotations:

^{2,5}A_{GlcA}Z a1D-GlcA,p/#acleavage_2_5--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd ^{1,4}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_1_4--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-2H,0,freeEnd
382.8026 31.1
383.1725 792.0
385.2049 350000.0
386.1978 42400.0

Annotations:

^{0,3}A_{GlcA}Y a1D-GlcA,p/#acleavage_0_3--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd
387.3209 33.0

Annotations:

freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S/#lcleavage)--6?1S/#lcleavage$MONO,Und,-2H,0,freeEnd
396.1764 4850.0

Annotations:

C a1D-GlcA,p/#ccleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd CY b1D-GlcNAc,p/#ccleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd Y freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd
414.1342 794.0
421.1532 1710.0
421.6805 127000.0
422.17 42800.0
422.6655 20100.0
423.1631 4160.0
423.6621 976.0
429.7207 844.0
430.2238 431.0

Annotations:

^{1,5}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_1_5(--4a1D-GlcA,p)--6?1S$MONO,Und,-H,0,freeEnd ^{1,5}A_{GlcNAc}Y b1D-GlcNAc,p/#acleavage_1_5(--4a1D-GlcA,p--4b1D-GlcNAc,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd ^{1,5}A_{GlcA}Y a1D-GlcA,p/#acleavage_1_5--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd
438.1923 332.0

Annotations:

^{2,4}A_{GlcA} a1D-GlcA,p/#acleavage_2_4--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd ^{2,4}A_{GlcNAc}Y b1D-GlcNAc,p/#acleavage_2_4--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd
443.125 174.0
456.1167 133.0
456.5062 62.1
458.152 80300.0

Annotations:

Z freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd B a1D-GlcA,p/#bcleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-H,0,freeEnd BY b1D-GlcNAc,p/#bcleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd CZ b1D-GlcNAc,p/#ccleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd
458.6314 144.0
459.1363 7640.0
460.1346 3000.0
460.7007 261.0
468.6992 484.0
469.7269 188000.0
469.8865 2920.0
470.229 69900.0
470.3906 270.0
470.7139 25300.0
471.7119 644.0
476.1454 851.0

Annotations:

C a1D-GlcA,p/#ccleavage--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-H,0,freeEnd CY b1D-GlcNAc,p/#ccleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd Y freeEnd--?b1D-GlcNAc,p(--4a1D-GlcA,p--4b1D-GlcNAc,p/#ycleavage)--6?1S$MONO,Und,-H,0,freeEnd
477.1468 181.0
499.2377 2260.0
500.2391 277.0

Annotations:

^{2,4}A_{GlcNAc}Z b1D-GlcNAc,p/#acleavage_2_4--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S$MONO,Und,-H,0,freeEnd
500.6793 71.1
501.2382 146.0
508.6819 900.0
509.1861 5960.0

Annotations:

^{1,4}A_{GlcNAc}Y b1D-GlcNAc,p/#acleavage_1_4--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd
509.7844 627.0
510.7081 62700.0
543.2357 638.0
544.2379 104.0

Annotations:

^{2,5}A_{GlcNAc}Z b1D-GlcNAc,p/#acleavage_2_5(--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p/#zcleavage)--6?1S/#lcleavage)--6?1S/#lcleavage}--??1S$MONO,Und,-H,0,freeEnd
552.2065 78.3
554.2341 42800.0

Annotations:

B b1D-GlcNAc,p/#bcleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S/#lcleavage$MONO,Und,-H,0,freeEnd
555.751 17.7
556.2261 791.0
634.2045 40600.0

Annotations:

B b1D-GlcNAc,p/#bcleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-H,0,freeEnd
634.2755 106.0

Annotations:

B b1D-GlcNAc,p/#bcleavage--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-H,0,freeEnd
635.1959 5340.0
636.1945 2030.0
637.0421 9.57
637.1913 216.0
746.3329 59.3
764.3569 1010.0
764.7819 13.2
765.3606 511.0

Annotations:

^{1,4}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_1_4--4a1D-GlcA,p--4b1D-GlcNAc,p(--4a1D-GlcA,p)--6?1S$MONO,Und,-H,0,freeEnd
766.3596 95.5
767.3591 36.9

There are 2 similar spectra:

Id Structure Score

5326

0.81

5320

0.54

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 934.15
(Calculated Monoisotopic)

Reducing End: Free reducing end

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • gradient with 0.1 ml/min run rate
  • water
  • water

Mass Spec

Waters Synapt G2-S HDMS

MIRAGE Structural data

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