Structure

    redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd

Core-2 O-glycans are required for galectin-3 interaction with the synovial glycoprotein lubricin

GlyTouCan Accession Number: Loading...

Peak Intensity
243.72 0.8

Annotations:

^{0,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
244.28 0.0

Annotations:

^{0,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
244.81 0.0
245.36 0.0
246.02 1.6
246.6 0.0
247.18 0.0
247.69 0.0
248.26 0.0
248.77 0.0
249.34 0.0
249.84 0.7
250.41 0.0
250.95 0.0
251.48 0.0
252.03 0.0
252.55 0.0
253.11 0.0
253.63 0.0
254.19 0.0
254.7 0.0
262.07 56.6

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{1,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_3--3b1D-Gal,p$MONO,Und,-H,0,redEnd
262.76 3.3
263.29 2.6
263.86 0.0
264.37 0.0
264.94 0.0
265.45 0.0
266.02 0.0
266.53 0.0
267.08 0.0
267.61 0.0
268.14 0.0
276.0 0.6

Annotations:

^{3,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_3_5--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
276.54 0.0

Annotations:

^{3,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_3_5--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
277.1 0.0
277.62 0.0
278.18 0.0
278.7 0.0
279.24 0.0
279.78 0.0
280.31 0.0
280.85 0.0
292.41 1.2

Annotations:

^{0,3}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_3--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p$MONO,Und,-H,0,redEnd
292.96 0.0
293.52 0.0
294.04 0.0
294.6 0.0
295.12 0.0
295.68 0.0
296.2 0.0
296.74 0.0
297.27 0.0
303.82 34.6
304.34 2.7
304.96 2.0
305.53 0.0
306.09 0.0
306.61 0.0
307.15 0.0
307.69 0.0
308.22 0.0
308.78 0.0
309.3 0.0
309.85 0.0
311.73 0.7
312.29 0.0
312.56 1.8
313.11 0.0
313.63 0.0
314.02 1.0
314.55 0.0
315.11 0.0
315.63 0.0
316.04 1.7
316.43 1.9
316.99 0.0
317.53 0.0
318.06 0.0
318.61 0.0
319.13 0.0
319.69 0.0
320.21 0.0
320.77 0.8
321.04 2.6
321.73 6.9
322.41 0.0
323.0 1.2
323.59 0.0
324.13 3.1
324.69 0.0
325.0 3.4
325.54 0.0
326.1 0.0
326.62 0.0
327.19 0.0
327.7 0.0
328.25 0.0
328.78 0.0
329.32 0.0
329.86 0.0
332.8 0.8
333.36 0.0
334.01 3.9
334.61 0.0
335.14 0.0
335.69 0.0
336.22 0.0
336.77 0.0
337.29 0.0
337.86 0.0
338.37 0.0
338.93 0.0
344.58 0.7
344.97 1.1
345.53 0.0
346.09 0.0
346.61 0.0
347.25 2.2
347.79 0.0
348.35 0.0
348.87 0.0
349.44 0.0
349.95 0.0
350.5 0.0
351.03 0.0
351.57 0.0
352.11 0.0
353.2 1.0
353.77 0.0
354.29 0.0
354.85 0.0
355.37 0.0
355.94 0.0
356.45 0.0
357.0 0.0
357.53 0.0
360.75 2.2
361.31 0.0
362.2 0.8
362.77 0.0
363.29 0.0
363.85 0.0
364.37 0.0
364.88 2.9
365.44 0.0
366.09 0.5

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
366.64 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
367.19 0.0
367.72 0.0
368.27 0.0
368.79 0.0
369.35 0.0
369.87 0.0
370.44 0.0
370.92 2.8
371.46 0.0
372.02 0.0
372.54 0.0
373.09 0.0
373.62 0.0
374.16 0.0
374.7 0.0
375.23 0.0
375.78 0.0
381.84 1.1
382.36 0.0
382.9 0.0
383.44 0.0
384.12 6.4

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
384.73 0.0
385.25 0.0
385.81 0.0
386.32 0.0
386.89 0.0
387.4 0.0
387.96 0.0
388.72 16.3

Annotations:

ZZ redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
389.11 62.3

Annotations:

ZZ redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
390.17 5.6
390.69 0.0
390.93 0.0
391.42 0.0
391.77 0.0
392.01 0.0
392.51 0.0
392.86 0.0
393.08 0.0
393.59 0.0
393.95 0.0
394.14 0.0
394.67 0.0
395.05 0.0
395.6 0.9
396.15 0.0
396.69 0.0
397.22 0.0
397.77 0.0
398.29 0.0
398.86 0.0
399.37 0.0
399.94 0.0
400.44 0.0
405.12 17.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
405.74 0.0
407.06 64.4

Annotations:

ZY redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
408.07 3.1
408.38 0.6
408.95 0.0
409.51 0.0
410.03 0.0
410.56 0.0
411.11 0.0
411.63 0.0
412.19 0.0
412.71 0.0
422.65 2.2
423.2 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
423.76 0.0
424.28 0.0
424.82 0.0

Annotations:

YY redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
425.35 0.6

Annotations:

YY redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
425.9 0.0
426.44 0.0
426.97 0.0
427.52 0.0
428.05 0.0
428.61 0.0
429.12 0.0
429.69 0.0
430.19 0.0
436.49 1.1
437.04 0.0
437.61 0.0
438.12 0.0
438.69 0.0
439.2 0.0
439.79 0.7
440.35 0.0
440.87 0.0
441.44 0.0
441.95 0.0
442.5 0.0
443.03 0.0
443.57 0.0
444.11 0.0
444.62 0.0
446.94 1.5
447.5 0.0
448.03 0.0
448.57 0.0
449.11 0.0
449.64 0.0
450.19 0.0

Annotations:

^{2,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_5(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
450.71 0.0
451.27 0.0
451.78 0.0
455.08 1.4
455.63 0.0
456.19 0.0
456.87 1.2
457.44 0.0
457.96 0.0
458.52 0.0
459.04 0.0
459.6 0.0
460.12 0.0
460.68 0.0
461.2 0.0
464.92 9.9

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
465.55 0.0

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
466.07 0.0

Annotations:

^{0,2}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
466.61 0.0

Annotations:

^{0,2}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
467.36 1.1
467.94 0.0

Annotations:

^{2,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_5(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
468.45 0.0

Annotations:

^{2,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_5(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
469.02 0.0
469.53 0.0
470.08 0.0
470.61 0.0
471.15 0.0
471.69 0.0
479.2 2.4

Annotations:

^{3,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_3_5--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
479.76 0.0
480.28 0.0
480.82 0.0
481.36 0.0
481.89 0.0
482.44 0.0
482.97 0.0
483.52 0.0
483.85 0.8

Annotations:

^{0,2}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
484.37 0.0

Annotations:

^{0,2}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
484.94 0.0
485.45 0.0
486.02 0.0
486.53 0.0
487.08 0.0
487.61 0.0
488.15 0.0
488.69 0.0
490.32 1.0
490.87 0.0
491.44 0.0
491.95 0.0
492.51 0.0
493.03 0.0
493.57 0.0
494.11 0.0
494.64 0.0
495.19 0.0

Annotations:

^{0,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_3--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
502.23 2.5
502.78 0.0
503.31 0.0
503.86 0.0
504.39 0.0
504.94 0.0
505.46 0.0
506.02 0.0
506.74 0.5
507.28 0.0
507.87 3.3
508.89 12.0
509.12 9.0
509.79 0.0
510.19 0.8
510.74 0.0
511.28 0.0
511.81 0.0
512.36 0.0
512.88 0.0
513.44 0.0
514.14 0.6
514.69 0.0
515.13 0.7
515.69 0.0
516.21 0.0
516.77 0.0
517.29 0.0
517.85 0.0
518.37 0.0
518.94 0.0
519.45 0.0
527.01 4.9
527.71 0.0
528.06 0.6
528.61 0.0
529.15 0.0
529.69 0.0
530.22 0.0
530.78 0.0
531.3 0.0
531.9 1.7
532.21 0.8
532.77 0.0
533.29 0.0
533.86 0.0
534.37 0.0
534.94 0.0
535.45 0.0
536.02 0.0
536.53 0.0
545.17 0.7
545.73 0.0
546.28 0.0
546.8 0.0
547.36 0.0
547.88 0.0
548.44 0.0
548.95 0.0
549.68 1.0
550.15 5.7
551.24 3.8
551.92 1.0
552.46 0.0
553.02 0.0
553.54 0.0
554.1 0.0
554.62 0.0
555.19 0.0
555.7 0.0
556.25 0.0
556.77 0.0
559.53 0.5
560.1 0.0
560.62 0.0
561.17 0.0
562.15 1.7
562.71 0.0
563.27 0.0
563.78 0.0
564.13 2.8
564.69 0.0
565.21 0.0
565.77 0.0
566.29 0.0
566.85 0.0
567.37 0.0
568.04 42.9
568.28 34.7
568.99 57.3

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
569.48 2.0

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
569.92 3.0
570.52 0.0
571.04 0.0
571.61 0.0
572.12 0.0
572.69 0.0
573.2 0.0
573.65 2.8
573.96 5.0
574.24 1.2
574.94 0.7
575.52 0.0
576.04 0.0
576.6 0.0
577.12 0.0
577.66 0.0
578.2 0.0
578.73 0.0
579.28 0.0
579.79 0.0
582.26 3.3
582.8 0.0
583.36 0.0
583.88 0.0
584.44 0.0
584.95 0.0
585.99 3.1
586.45 0.8
587.15 24.6

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
588.13 4.4
588.69 0.0
589.27 0.0
589.78 0.0
590.35 0.0
590.86 0.0
591.43 0.0
592.11 3.9
592.73 0.0
593.23 0.0
593.81 0.0
594.3 0.0
594.83 0.0
595.37 0.0
595.91 0.0
596.49 0.0
596.98 0.0
606.56 1.5
607.12 0.0
607.66 0.0
608.2 0.0
608.66 0.4
610.04 643.7

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
611.2 42.4
611.85 0.0
612.37 0.0
612.94 0.0
613.45 0.0
614.01 0.0
614.53 0.0
615.07 0.0
615.61 0.0
616.14 0.0
626.8 1.5
627.36 0.0
628.23 54.3

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
628.89 11.6
629.1 9.2
629.32 2.4
629.9 0.0
630.44 0.0
630.97 0.0
631.53 0.0
632.05 0.0
632.61 0.0
633.12 0.0
633.69 0.0
634.19 0.5
634.76 0.0
635.28 0.0
635.82 0.0
636.36 0.0
636.89 0.0
637.44 0.0
637.97 0.0
638.52 0.0
639.03 0.0
656.35 1.3
657.18 3.0
657.76 0.0
658.28 0.0
658.83 0.0
659.36 0.0
659.89 0.0
660.44 0.0
660.97 0.0
661.52 0.0
662.04 0.0
666.73 0.4
667.27 0.0
667.78 0.0
668.34 0.0
669.02 0.9
669.59 0.0
670.18 2.5
670.73 0.0
671.28 0.0

Annotations:

^{2,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_5(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
671.8 0.0
672.36 0.0
672.97 1.8
673.53 0.0
674.06 0.0
674.61 0.0
675.14 0.8
675.69 0.0
676.22 0.0
676.77 0.0
677.29 0.0
677.86 0.0
678.37 0.0
678.94 0.0
679.45 0.0
682.25 2.0
682.8 0.0
683.36 0.0
683.91 1.0
684.45 0.0
684.98 0.0
685.53 0.0
686.06 0.0
686.61 0.0
687.13 0.0

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
687.69 0.0

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
688.21 0.0
688.77 0.0
689.44 0.7
690.02 0.0
690.53 0.0
690.87 0.0
691.1 0.0
691.61 0.0
691.95 0.0
692.18 0.0
692.69 0.0
693.03 0.0
693.26 0.0
693.77 0.0
699.05 1.2
699.61 0.0
700.15 0.0
700.69 0.0
701.22 0.0
701.93 2.1
702.19 1.0
702.76 0.0
703.28 0.0
703.82 0.0
704.36 0.0
704.89 0.0
705.44 0.0
705.97 0.0
706.52 0.0
707.03 0.0
726.46 1.8
726.89 0.7
727.44 0.0
727.97 0.0
728.52 0.0
728.82 3.1
729.94 43.3
730.26 16.4
730.9 11.9
731.28 3.1
731.87 0.0
732.43 0.0
732.95 0.0
733.29 0.0
733.52 0.0
734.22 0.6
734.77 0.0
735.29 0.0
735.85 0.0
736.37 0.0
736.92 0.0
737.45 0.0
737.98 0.0
738.53 0.0
744.97 3.2
745.53 0.0
745.93 2.1
746.49 0.0
747.03 0.0
748.0 30.8
748.33 8.2
749.03 8.6
749.27 9.6
749.86 0.0
750.38 0.0
750.94 0.0
751.46 0.0
752.02 0.0
752.54 0.0
753.1 0.0
753.62 0.0
754.16 0.0
754.93 5.9
755.49 0.0
756.0 1.7
756.54 0.5
757.11 0.0
757.64 0.0
758.19 0.0
758.71 0.0
759.27 0.0
759.79 0.0
760.35 0.0
760.87 0.0

There are 2 similar spectra:

Id Structure Score

1.00

4262

0.98

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 789.32
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

Report errors in this entry

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