Structure

    redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
299.0 1.1
304.07 1.8
317.18 5.3
322.02 1.9
364.25 4.8

Annotations:

B a1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
365.37 2.4
369.37 1.9
387.2 15.8

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
389.26 3.6

Annotations:

ZZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
405.18 27.2

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
407.11 18.3

Annotations:

YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
409.11 1.1
425.34 1.3

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
431.12 1.6
490.03 1.5
527.13 3.1
527.39 3.7

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
531.33 2.5
543.38 1.1
550.38 2.9
566.89 1.1
567.33 2.1

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
567.83 1.2

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
568.27 5.9

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
568.5 2.3
587.23 15.9

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
592.26 5.7
610.27 10.2

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
612.46 2.1
627.76 1.2
628.2 8.9

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
628.51 2.5
629.09 4.0
640.28 1.2
643.28 1.1
649.12 1.5
670.32 1.7
676.2 1.5
682.22 6.3
685.52 1.2
688.27 4.4
699.31 4.2
700.13 7.7
700.43 1.8
701.24 1.1
705.82 1.3
711.64 1.3
712.33 1.3
716.01 6.7
716.39 3.3
717.35 1.5
724.32 1.6
730.35 59.2

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
730.83 1.7
733.29 3.3
735.33 1.6
738.33 1.2
740.46 1.3
748.52 2.2
753.33 9.0
754.26 1.6
770.28 45.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
771.26 1.4
771.77 1.9
772.3 16.9

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
773.35 1.5
776.31 1.4
790.43 27.8

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
791.33 9.0
820.33 1.7
843.33 4.2
843.74 2.1
856.45 1.7
857.44 1.2
873.17 10.2
873.61 3.0
874.07 2.6
874.57 2.0
885.31 16.6
885.59 7.4
886.26 2.3
890.83 2.3
891.48 12.2
894.5 2.3
895.23 3.1
902.73 1.2
903.62 4.8
927.05 1.2
930.22 1.3
931.33 1.1
933.33 324.8
934.48 17.2
951.29 17.6
951.59 4.9
952.28 2.9
956.77 4.7
957.17 2.0
973.17 5.3
984.4 1.6
997.57 2.2

There are 2 similar spectra:

Id Structure Score

4872

0.52

0.52

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 992.40
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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