Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
290.12 117.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.1 9.3
308.06 11.4

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
348.32 3.0

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.15 22.0

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
371.07 3.0
384.34 46.3

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
385.18 3.7
417.03 3.3
419.36 4.2
435.27 4.3
436.29 3.6

Annotations:

Z^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
451.28 4.5
452.95 3.5
453.25 5.2

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
454.27 12.2

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
455.23 2.8
461.22 9.9
477.18 22.4
478.01 5.3
478.32 5.1
479.21 14.8
479.6 3.0
480.26 2.9
493.2 3.9
495.26 15.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
503.04 3.3
507.19 10.4
513.27 16.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.09 4.9
514.41 2.9
521.22 23.3
522.25 2.6
555.19 2.6
569.37 8.0

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
597.28 77.4
598.19 12.5
598.45 7.5
599.23 2.7
615.25 152.5
616.28 34.5
631.34 3.9
638.28 2.4
639.25 6.7

Annotations:

Z^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
640.1 3.4
656.2 2.5
657.25 209.2

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.27 37.3
674.23 3.4
675.25 214.6

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.3 42.8
692.08 7.8
692.41 6.3
693.37 2.8
697.2 2.8
710.38 4.0
715.46 6.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
716.26 3.4
733.29 42.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
734.34 10.0
752.39 10.5
782.34 12.3
782.77 2.5
785.45 19.8
794.29 25.0
795.32 6.1
800.3 6.1
801.03 3.3
803.34 94.2

Annotations:

^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
804.42 18.2
824.3 4.0
826.45 5.6
842.23 17.7
842.52 9.1
843.27 9.9
857.42 2.6
860.41 5.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.19 5.2
861.5 6.1
862.57 3.8
868.11 3.4
878.41 14.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.58 9.4
880.29 3.8
886.31 8.0
896.55 2.5
904.32 4.5
916.31 10.7
917.22 7.0
922.5 2.4
928.39 2.4
929.25 3.1
937.29 3.0
946.37 199.9
947.38 72.9
964.34 655.3
965.35 214.8
982.18 3.1
988.49 11.6
989.45 7.6

There are 8 similar spectra:

Id Structure Score

4874

0.99

5447

0.97

6816

0.97

5737

0.87

5800

0.85

5801

0.82

5589

0.76

5736

0.72

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1023.39
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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