Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
324.71 0.5

Annotations:

C b1D-GlcNAc,p/#ccleavage--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
366.09 0.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
367.05 0.7
367.34 0.2
384.25 0.2

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
389.12 2.6

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
390.05 0.7
407.09 0.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
407.31 0.3
408.64 0.6
409.05 2.3
423.75 0.2

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
478.71 0.2
479.94 0.1
491.18 0.4
509.2 0.4

Annotations:

^{2,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
510.17 1.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
527.06 0.2

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
528.09 0.2

Annotations:

C ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
545.56 0.3
551.19 1.1

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.15 4.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.8 0.0
570.22 2.6

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
586.88 0.2
587.17 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
612.32 0.2
625.32 0.3
631.2 0.2
649.24 0.2
673.08 2.6

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
673.45 0.7
674.2 0.9
687.19 0.4
691.29 1.3
691.92 0.2
692.74 0.2
706.48 0.4
706.84 0.3
712.09 2.0
712.42 0.1
713.35 0.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
715.2 26.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
716.22 3.7
730.25 0.5
730.85 0.6

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
731.2 0.3
732.11 0.3
733.05 5.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
733.34 2.2
734.23 1.3
748.91 0.3
749.35 0.4

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
750.08 0.3
750.48 0.2
754.44 0.7
772.17 11.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
773.12 1.8
773.35 0.7
785.15 0.1
790.07 0.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
792.11 0.1
799.06 0.4
820.73 0.3
835.18 2.4
835.43 1.8
835.66 0.6
836.28 0.7
853.09 1.6
853.39 1.2
854.2 1.3
875.14 0.7
876.89 0.7
877.24 0.8

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
886.16 0.4
892.18 0.4
893.34 0.3
894.14 0.2
895.31 22.6

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
896.24 7.1
896.95 0.0
919.89 0.4
933.99 0.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
934.36 0.1
936.26 1.1
937.17 0.2
978.14 0.4
979.51 0.3
996.16 0.3
997.41 0.1
1024.38 0.2
1038.0 3.1
1038.34 4.8
1039.13 3.8
1039.37 4.3
1039.94 0.0
1056.41 3.6
1057.22 1.6
1066.62 0.2
1098.54 0.3

There are 8 similar spectra:

Id Structure Score

3772

0.91

5525

0.91

0.76

4888

0.76

3985

0.72

5814

0.66

5602

0.54

7906

0.54

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1097.43
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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