Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
340.89 0.3
347.26 0.2

Annotations:

Z^{2,4}X_{GlcNAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd
365.2 0.4

Annotations:

Y^{2,4}X_{GlcNAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd
366.13 0.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
381.94 0.7

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd CY ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
382.17 0.4
389.26 0.2

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
406.99 1.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
408.27 0.2
424.3 0.3

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
424.99 0.3

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
431.85 0.2
436.86 0.2
466.0 0.2
467.04 0.3

Annotations:

Y^{0,2}X_{GlcNAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
479.14 0.3
506.94 0.2
509.1 1.4

Annotations:

Z^{2,4}X_{GlcNAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd ^{2,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
510.1 0.2
526.81 0.7
527.26 1.7

Annotations:

Y^{2,4}X_{GlcNAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd ^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
527.57 0.2

Annotations:

^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
528.08 0.8
545.38 0.4
551.18 1.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
567.17 0.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
569.14 10.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.14 1.3
570.42 0.5
587.14 2.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
587.41 0.7
626.98 0.5
627.38 0.7

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
627.99 0.3
634.97 0.3
648.16 0.2
669.36 0.3
689.03 0.2
707.08 0.3
712.23 2.2
713.33 0.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
730.36 3.8

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}X_{GlcNAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd
730.65 0.6
731.18 2.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
731.54 0.4
732.14 0.3
748.11 2.1
748.44 0.8
749.18 3.9

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
750.19 0.7
754.02 0.4
754.46 0.6
755.17 0.4
772.19 41.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
773.24 12.6
790.19 8.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
790.81 0.3
791.21 2.9
794.25 0.2
802.1 0.3
830.2 0.4
831.29 0.2
856.19 0.3
857.19 0.3
874.2 0.6
874.44 0.3
892.22 8.0
893.25 0.9
893.49 0.3
898.26 0.5
910.11 2.4
910.37 2.0
910.76 0.2
911.34 1.9
932.05 0.2
933.79 0.3
934.18 3.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
934.5 0.7
935.29 0.3
935.76 0.2
952.25 36.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
953.23 13.0
953.7 0.9
973.91 0.2
995.31 0.6
1000.55 0.2
1035.26 1.0
1035.62 0.2
1053.09 0.7
1053.35 0.4
1076.93 0.6
1095.27 15.5
1096.44 4.8
1112.51 0.2
1113.35 6.3
1114.31 4.4
1126.1 0.2
1127.46 0.4
1156.17 1.5
1156.49 1.4

There are 8 similar spectra:

Id Structure Score

3774

0.89

6818

0.87

3775

0.82

3988

0.64

7909

0.55

8036

0.55

4273

0.54

8038

0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1154.45
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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