Structure

    redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd

The O-glycome of human nigrostriatal tissue and its alteration in Parkinsons disease

GlyTouCan Accession Number: Loading...

Peak Intensity
196.09 0.3
197.39 0.5
207.78 0.7
208.09 1.9
213.74 0.6
220.31 0.7
223.81 0.4
226.1 3.5
226.83 0.5
227.21 0.9
238.0 5.8
243.95 1.1
252.17 0.3
257.0 0.5
258.09 1.7
258.33 0.4
268.88 0.3
269.88 0.7
276.15 4.1
277.04 1.0
280.08 0.7
286.08 0.7
287.08 1.8
288.02 2.0
288.23 0.8
288.97 1.1
289.18 0.5
299.85 2.0
304.94 0.4
305.87 0.7
306.25 3.3
306.75 0.4
307.1 0.5
312.83 1.2
318.15 337.4
319.15 58.7
330.09 8.2

Annotations:

BZ ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
330.96 2.1
332.41 0.6
334.07 2.4
337.89 0.7
345.14 1.3
345.9 0.7

Annotations:

BZ ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
346.22 0.8

Annotations:

BZ ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
346.67 1.4
347.12 0.4
348.13 122.3

Annotations:

BY ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p/#ycleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd CZ ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
349.14 13.2
349.97 1.4
350.96 0.6
351.33 0.7
359.96 0.5
360.27 1.5
363.94 1.8

Annotations:

BY ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
364.57 1.0

Annotations:

BY ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
364.93 1.8
365.36 1.1
366.29 3.0

Annotations:

CY ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p/#ycleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#ycleavage)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.53 1.6

Annotations:

CY ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p/#ycleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#ycleavage)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
367.25 1.7

Annotations:

^{2,4}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_2_4(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
396.79 1.4
397.31 0.4
399.56 0.9
436.32 0.9

Annotations:

^{1,4}A_{Man}Z ?1D-Man,p/#acleavage_1_4--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
445.36 1.5
459.24 0.9
464.27 1.4

Annotations:

^{1,5}A_{Man}Z ?1D-Man,p/#acleavage_1_5--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
473.15 0.5
476.9 0.4
481.33 0.4
491.11 0.5
491.98 0.3
494.05 0.4
510.24 225.6

Annotations:

B ?1D-Man,p/#bcleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
511.25 63.8
512.24 3.2

Annotations:

Z redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#zcleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
513.29 2.5
515.29 1.4
524.21 1.7
528.07 1.1

Annotations:

C ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
528.48 2.0

Annotations:

C ?1D-Man,p/#ccleavage--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
529.0 1.2
530.3 2.4

Annotations:

Y redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p/#ycleavage)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
531.31 1.7
533.33 1.2
538.99 0.4
542.72 0.4
543.15 1.7
555.89 1.9
558.23 1.3
560.99 1.2
570.4 0.5

Annotations:

^{1,3}A_{Man} ?1D-Man,p/#acleavage_1_3--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
575.45 0.8
577.01 1.5
593.06 0.3
595.35 0.5
617.37 0.4
620.96 1.3
632.37 4.2
632.64 0.8

There are 1 similar spectra:

Id Structure Score

1.00

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 691.27
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Homo Sapiens
Tissue - Brain

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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