Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-2H,0,redEnd

Sulfation of O-glycans on mucin-type proteins from serous ovarian epithelial tumors

GlyTouCan Accession Number: Loading...

Peak Intensity
282.1 0.3

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1S$MONO,Und,-H,0,redEnd
292.98 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S/#lcleavage$MONO,Und,-2H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage$MONO,Und,-2H,0,redEnd
296.99 0.0
299.09 0.0
302.03 0.1
307.01 0.1
307.78 0.1
312.06 0.1
317.25 0.1
321.02 0.1
322.18 0.1
325.72 0.1
331.28 0.0
336.91 0.1
340.92 0.0
342.55 0.1
343.12 0.1
348.14 0.1
351.64 0.1
352.11 0.0
352.41 0.1
357.24 0.1
363.99 0.1

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1S$MONO,Und,-2H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-2H,0,redEnd B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
372.09 0.0
373.06 3.1

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S$MONO,Und,-2H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-2H,0,redEnd
373.56 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S$MONO,Und,-2H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-2H,0,redEnd
373.89 0.1

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage$MONO,Und,-2H,0,redEnd
377.06 0.1
378.14 0.1
379.26 0.1
381.98 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
383.56 0.0
384.99 0.1
390.14 0.1
392.41 0.1
392.72 0.0
393.81 0.0
394.78 0.1
396.2 0.1
397.11 0.0
402.34 0.0
403.07 0.1
405.28 0.1
405.95 0.1
409.25 0.1
411.9 0.1
412.86 0.2
413.31 0.1
413.64 0.1

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-2H,0,redEnd
414.17 0.1

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-2H,0,redEnd
415.54 0.1
418.86 0.1
419.12 0.1
421.28 0.1
422.04 0.0
425.96 0.1
427.17 0.1
431.04 0.1
431.49 0.1
432.28 0.2
433.04 0.2
435.41 0.0
439.4 0.1
444.07 10.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-H,0,redEnd
445.04 1.0
445.79 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
448.59 0.0
449.15 0.3
449.96 0.5
450.25 0.2
450.58 0.1
452.33 0.1
453.2 0.2
454.09 156.4

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}(--??1S)--??1S$MONO,Und,-2H,0,redEnd
454.59 19.9

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}(--??1S)--??1S$MONO,Und,-2H,0,redEnd
456.94 0.0
458.82 0.0
461.96 0.1

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-H,0,redEnd
463.19 0.1
464.08 21.7

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
465.11 1.3
466.29 0.1
521.69 0.0
524.03 3.8

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-H,0,redEnd
524.89 0.6
543.99 0.8
545.06 0.1
605.04 0.0
607.29 0.2
649.19 0.1

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-H,0,redEnd
666.17 0.1
667.22 2.5

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S/#lcleavage}--??1S$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??1S/#lcleavage}--??1S$MONO,Und,-H,0,redEnd
667.78 0.1
668.27 0.3
728.63 0.0
745.81 0.1
746.7 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-H,0,redEnd
747.12 0.7

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1S$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p--??1S)--??1S$MONO,Und,-H,0,redEnd
748.17 0.1
811.26 0.2

There are not related spectra

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 988.16
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Homo Sapiens
Tissue - Cyst Fluid

HPLC

Column

Method

  • 100 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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