Structure

    redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
370.26 1.0
370.81 0.0
389.47 0.8
390.03 0.0
404.96 0.5

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
406.29 0.5
406.89 1.1
407.26 2.0
412.98 0.6
413.53 0.0
425.32 1.3
425.87 0.0
437.0 0.6
437.54 0.0
449.58 0.7
450.13 0.0
480.13 0.6
480.69 0.0
495.39 0.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
495.95 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
508.29 1.5
508.85 0.0
513.57 0.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.12 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.51 0.4
527.11 0.3
566.97 0.8

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
567.41 0.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
567.95 0.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
568.56 1.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.16 0.0

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
587.18 0.7

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
587.68 0.4

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
615.45 1.3
616.14 0.4
628.48 2.8

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
629.32 1.1
629.87 0.0
640.54 1.4
641.11 0.0
657.19 2.4
657.74 0.0
669.43 0.5
676.37 1.1
676.94 0.0
679.44 1.4

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
680.02 0.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
708.59 0.7
709.14 0.0
717.58 1.1
718.12 0.0
730.24 6.3
730.5 1.7
730.81 2.1
731.39 1.5
731.94 0.0
733.36 0.5
737.87 1.4
738.58 3.3
739.34 1.3
739.94 0.0
746.43 0.8
746.97 0.0
748.07 1.2
748.5 2.7
749.05 0.0
758.04 2.0
758.6 0.0
771.83 0.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
782.5 0.8
783.04 0.0
786.31 1.0
786.87 0.0
787.49 0.4
790.47 20.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
791.52 7.0
792.11 0.0
795.44 1.6
796.02 0.0
800.03 1.1
800.56 0.0
818.37 2.7
818.94 0.0
819.47 0.6
820.02 0.0
843.64 0.4
850.23 1.8
850.78 0.0
859.5 0.8

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.19 3.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.69 2.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.27 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
877.96 1.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
878.48 2.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
878.8 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.47 0.7
880.03 0.0
914.8 0.8
915.36 0.0
936.46 0.3

There are 7 similar spectra:

Id Structure Score

0.86

6756

0.84

4882

0.84

0.84

5213

0.67

3898

0.64

5598

0.64

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1080.41
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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