Structure

    redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

400.0500.0600.0700.0800.0900.0m/z0.010203040Abundance
Peak Intensity
363.93 1.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd BY ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.52 1.2

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.32 2.5

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
433.2 0.5
451.37 0.6
484.81 0.5
493.47 0.5
495.44 2.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
501.34 0.8
509.12 1.3
510.13 0.6

Annotations:

B ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
527.51 5.9

Annotations:

C ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
528.49 1.0

Annotations:

C ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
549.25 0.4

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.25 15.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.16 0.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.53 1.5
587.36 38.5

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.16 4.8

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.47 3.5
597.2 0.9
615.44 0.9
622.14 0.5
629.28 0.6
638.18 1.2
639.33 2.0
640.36 0.4
645.4 0.6
656.04 1.3
657.02 0.8
664.23 3.8
664.67 1.1
665.31 1.5
675.5 1.2
682.35 9.1
683.19 1.1
694.53 0.6
697.29 0.8

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
698.03 0.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
698.31 0.5

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
698.68 0.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
699.45 0.8
706.36 3.4
712.51 0.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
714.31 0.5
716.12 1.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
716.53 0.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
717.07 1.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
717.47 1.2
720.56 0.6
723.98 0.7
724.52 3.2
725.34 0.7
770.73 0.4
772.26 0.8

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
772.8 0.6

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
773.35 0.9
774.59 1.1
775.42 0.6
782.52 0.5
790.16 1.2

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
790.58 1.1

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
800.38 8.1
801.55 0.5
818.42 11.9
819.46 5.5
822.25 1.2
822.54 0.5
836.39 1.1
840.38 0.8
841.33 1.2
858.3 4.7
859.39 1.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.6 7.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.04 1.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.37 5.0
863.34 0.9
876.3 1.0
876.61 1.9
877.47 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
878.44 32.5

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.62 4.9
894.13 0.4
895.5 0.7
900.35 0.4
906.45 1.5
913.28 0.6
913.55 0.7
917.91 0.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
918.55 5.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
919.26 1.2

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
921.54 0.6
926.43 0.9
930.49 0.7
935.22 1.6
936.47 20.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
937.36 8.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
937.59 6.6
937.82 0.9
938.46 1.4

There are not related spectra

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1226.47
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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