Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
380.57 0.7
381.12 0.0
451.22 0.4
451.78 0.0
513.31 0.6

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
513.86 0.0

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
514.25 0.3
514.8 0.0
519.35 2.2
519.91 0.0
520.25 0.7
520.79 0.0
537.39 3.6
537.97 0.0
538.57 0.0
539.43 1.0
539.98 0.0
543.97 0.4
544.53 0.0
563.31 0.7
563.87 0.0
567.35 0.7
567.9 0.0
579.15 3.9
579.54 0.7
580.27 0.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
580.53 0.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
581.27 46.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
581.78 1.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
582.2 5.4
582.47 3.5
583.05 0.0
583.47 0.9
584.03 0.0
591.35 0.7
591.88 0.0
597.41 1.2
597.96 0.0
598.56 0.5

Annotations:

C ?1D-GalNAc,p/#ccleavage--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
599.11 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
629.26 0.5
629.79 0.0
639.32 0.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
639.87 0.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
656.47 0.8

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
656.9 1.0

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
657.5 0.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
658.07 0.0

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
692.36 0.6
692.94 0.0
732.96 0.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
733.52 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
733.99 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
734.53 0.0
764.51 1.4
765.05 0.0
765.61 0.0
786.57 0.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
787.17 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
803.0 1.0
803.62 1.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
804.37 1.7

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
804.94 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
805.45 0.0
806.07 0.4
806.61 0.0
844.59 0.6
845.13 0.0
858.14 0.3
858.7 0.0
868.45 0.6
869.02 0.0
878.44 0.9

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
879.27 0.9

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
879.92 0.0
886.42 0.8
887.27 0.6
887.81 0.0
892.61 0.3
893.18 0.0
907.32 0.8
907.86 0.0
915.45 1.3
915.89 0.9
916.13 2.8
916.41 2.1
917.0 0.0
917.34 0.5
917.63 0.6
918.19 0.0
923.23 0.4
923.62 1.5
924.19 0.0
924.7 0.0
927.85 0.2
928.4 0.0
932.11 0.2
932.68 0.0
933.37 0.8
933.94 0.0

There are not related spectra

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1314.49
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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