Structure

    redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
191.7 2.7
192.27 0.0
192.79 0.0
201.91 14.3

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p$MONO,Und,-H,0,redEnd
202.15 3.5

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
202.71 0.0

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
203.27 0.0

Annotations:

^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
204.11 25.6

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
204.7 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
205.25 0.0
205.78 0.0
220.81 1.3

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
221.37 0.0

Annotations:

^{2,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
222.36 6.8

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
222.95 0.0

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
223.52 0.0
224.03 0.0
228.02 7.9
228.65 0.0
229.19 0.0
229.72 0.0
246.3 3.1

Annotations:

^{2,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_5--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
246.86 0.0

Annotations:

^{2,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_5--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
247.4 0.0

Annotations:

^{2,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_5--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
276.25 4.9
276.79 0.0
277.36 0.0
277.87 0.0
321.95 3.6
322.52 0.0
323.03 0.0
323.59 0.0
346.32 1.8
346.87 0.0
347.39 3.4
347.95 0.0
348.49 0.0
349.03 0.0
365.32 1.8

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.87 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.44 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
384.3 22.6

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.94 0.0

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
385.33 7.8
385.95 0.0
386.49 0.0
387.03 0.0
407.16 4.8
407.69 5.9
408.33 3.5
408.88 0.0
409.44 0.0
409.96 0.0
437.41 3.1
437.94 0.0
438.24 2.0
438.78 0.0
439.31 0.0
446.22 2.5
446.78 0.0
447.3 0.0
479.56 17.7
480.33 9.1
480.94 0.0
481.47 0.0
482.02 0.0
488.32 2.2
488.87 0.0
489.41 0.0
513.45 4.8
513.99 0.0
514.53 0.0
515.06 0.0
527.46 8.3
528.11 1.1
528.68 0.0
529.2 0.0
539.3 3.1

Annotations:

^{1,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_5--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
539.85 0.0

Annotations:

^{1,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_5--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
540.26 8.0
540.8 0.0
541.36 0.0
541.88 0.0
543.09 7.2
543.63 0.0
544.19 0.0
544.5 1.8
545.32 6.0
545.9 0.0
546.41 3.7
546.96 0.0
547.52 0.0
548.04 0.0
558.14 2.2
558.69 0.0
559.22 0.0
1014.4 6.3
1014.95 0.0
1015.49 0.0
1016.03 0.0

There are 2 similar spectra:

Id Structure Score

0.58

4834

0.58

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 586.24
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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