Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
288.26 1.0
290.14 15.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.17 0.9
308.13 1.9

Annotations:

C ?1D-GalNAc,p/#ccleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
348.27 1.1

Annotations:

B ?1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
371.41 1.1
384.23 9.7

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.49 3.8

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
408.53 0.8
417.29 0.9
432.18 1.3
435.1 0.9
454.19 1.4
459.39 1.7
477.06 1.5
477.36 1.3
479.3 5.3
479.63 0.9
493.28 0.9
495.22 4.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
496.12 3.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
496.43 0.8
507.54 1.5
513.1 3.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.33 1.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
515.44 1.4
521.42 2.6
522.5 1.3
527.2 1.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
529.3 1.4
597.33 15.0
597.65 2.9
598.28 5.4
598.67 0.8
615.4 18.7
616.42 2.8
625.6 1.3
636.75 1.2
657.28 31.5

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
657.65 5.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.12 1.8

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.45 6.4
674.81 1.1

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
675.39 38.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.66 3.5
677.38 1.2
692.41 3.1
709.75 0.9
715.14 1.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
715.5 1.6

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
716.01 1.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
716.31 1.1
732.27 0.9
733.14 3.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
733.65 7.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
734.36 5.4
734.66 1.3
752.37 4.2
753.65 1.1
764.62 0.9
769.45 1.1
783.38 1.4
785.59 6.9
794.22 2.7
794.44 2.4
794.68 1.2
803.27 6.6
803.56 5.4
803.81 1.7
804.5 2.7
826.57 1.9
842.51 3.7
860.08 1.5

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
862.58 2.2
869.12 1.7
878.25 2.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
878.65 1.5

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.66 3.3
880.15 1.6
885.76 0.8
886.53 1.9
899.44 0.9
905.01 1.2
917.48 2.0
933.45 1.0
946.47 44.5
947.4 13.6
963.48 2.0
964.58 107.1
965.49 51.7
982.21 2.3
982.49 4.3
986.4 1.1
987.47 2.7
988.11 1.5
988.71 0.9
989.42 1.2
1079.76 1.1
1723.16 1.1
1959.03 0.8

There are 9 similar spectra:

Id Structure Score

4874

0.98

0.98

5447

0.97

6816

0.95

5800

0.85

5801

0.83

5589

0.76

5736

0.64

3892

0.53

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1023.39
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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