Colorectal cancer cell lines show striking isomeric diversity of their O-glycome

Katarina Madunic, Tao Zhang, Oleg A. Mayboroda, Stephanie Holst, Kathrin Stavenhagen, Chunsheng Jin, Niclas Karlsson, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer, Manuscript

Retention Time Recorded Precursor Mass Structure
28.1 966.37 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
32.13 749.32 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
44.2 1040.39 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
46.0 895.37 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--2a2L-Fuc,p@270$MONO,Und,-H,0,redEnd
48.8 1114.41 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3b1D-GlcNAc,p--?b1D-Gal,p)--6b1D-GlcNAc,p--?b1D-Gal,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Homo Sapiens
Tissue - Cell Line
Cell line - SW480 cell

HPLC

Column Hypercarb

Method

  • 73 min gradient with 0,6ul/min run rate
  • 10mM ammonium bicarbonate
  • 60% ACN, 10mM ammonium bicarbonate

Mass Spec

Bruker Daltonik amaZon Speed ETD ETD

MIRAGE

See report

UniCarb-DR Creative Commons License

Supported by SIB ExPASy  |  JST / NBDCDICP