Binding of Helicobacter pylori to mucin-type fusion proteins carrying Leb, sLex, LDN and GlcNAcα4Gal determinants on different O-glycan core chains

Yolanda H. Mthembu, Johan Olofsson Edlund, Chunsheng Jin, Reeja M. Cherian, Jining Liu, Niclas G. Karlsson, Thomas Borén, and Jan Holgersson, Manuscript

Retention Time Recorded Precursor Mass Structure
6.86 384.17 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
8.67 587.17 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--4a1D-GlcNAc,p$MONO,Und,-H,0,redEnd
8.82 675.25 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
9.56 733.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p(--3b1D-Gal,p)--4a1L-Fuc,p$MONO,Und,-H,0,redEnd
9.62 895.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p(--4a1L-Fuc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
9.8 587.08 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
10.11 879.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--4a1L-Fuc,p$MONO,Und,-H,0,redEnd
10.11 675.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
10.16 1024.17 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--4a1L-Fuc,p$MONO,Und,-H,0,redEnd
10.41 749.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
10.44 895.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
10.84 1024.42 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p(--4b1D-Gal,p--3a2D-NeuAc,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
10.9 587.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
10.9 1041.42 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--4a1L-Fuc,p$MONO,Und,-H,0,redEnd
11.03 749.33 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
11.18 691.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuGc,p$MONO,Und,-H,0,redEnd
11.22 733.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--3b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
11.45 1244.42 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p(--3b1D-Gal,p--3b1D-GlcNAc,p(--3b1D-Gal,p)--4a1L-Fuc,p)--4a1L-Fuc,p$MONO,Und,-H,0,redEnd
12.1 1406.42 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p(--4b1D-Gal,p--3b1D-GlcNAc,p(--3b1D-Gal,p)--4a1L-Fuc,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
12.27 966.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.57 878.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
12.68 982.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuGc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.35 1186.33 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p(--4b1D-Gal,p--3a2D-NeuAc,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
13.49 1186.17 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--4b1D-Gal,p--3a2D-NeuAc,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
13.5 1081.25 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
15.16 878.33 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.17 1040.25 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
15.19 1260.33 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--3b1D-GlcNAc,p(--3b1D-Gal,p)--4a1L-Fuc,p$MONO,Und,-H,0,redEnd
16.29 1040.5 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.87 878.33 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.14 738.26 [M -2H]2- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p(--3a1L-Fuc,p)--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-2H,0,redEnd
18.6 1040.42 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.79 530.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
19.11 733.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
19.63 895.25 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
21.53 1041.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
21.63 1331.25 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column Hypercarb

Method

  • 46 min gradient with 0.010 mL/min run rate
  • 10 mM NH4HCO3
  • 0-45% acetonitrile in 10 mM NH4HCO3

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

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