Binding of Moraxella catarrhalis to mucins from the lower airways of tobacco smokers and patients with pneumonia or COPD is dependent on sialic acid

M Padra, J Benktander, JT Padra, A Andersson, B Levänen, S Tengvall, K Christensson, I Qvarfordt, R Gad, M Paulsson, A Lindén and SK Lindén, Manuscript

Retention Time Recorded Precursor Mass Structure
10.05 513.23 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
10.57 587.26 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
10.85 587.26 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--4a1D-GlcNAc,p$MONO,Und,-H,0,redEnd
10.88 675.26 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
11.2 716.36 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p)--6b2D-NeuAc,p$MONO,Und,-H,0,redEnd
11.72 587.33 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
12.45 790.39 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4a1D-GlcNAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
12.75 675.26 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.78 749.45 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
13.11 1024.41 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.14 878.33 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4a1D-GlcNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.51 749.39 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
13.61 716.36 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.27 966.5 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.29 733.24 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p(--3a1D-GalNAc,p)--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
14.73 1301.59 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p(--3a1D-GalNAc,p)--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
15.02 1155.47 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
15.51 1040.49 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.72 1186.49 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--??1D-Gal,p--3a2D-NeuAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
17.66 1331.43 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.96 1477.55 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p--3a2D-NeuAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
19.07 1098.38 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
22.24 733.24 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
22.91 1098.38 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p)--6b1D-GlcNAc,p--3b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
23.67 1331.43 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
24.6 1041.49 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
25.79 821.45 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Homo Sapiens
Tissue - Mucus

HPLC

Column Hypercarb

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

UniCarb-DR Creative Commons License

Supported by SIB ExPASy  |  JST / NBDCDICP