A-series gangliosides inhibit TGF-β-induced epithelial-mesenchymal transition via TRI degradation

Jing Zhang, Jin Ma, Hailiang Mei, Cedrick C. Agaser, Birol Cabukusta, Katarina Madunic, Gerard van der Zon, Manfred Wuhrer, Tao Zhang and Peter ten Dijke, Manuscript

Retention Time Recorded Precursor Mass Structure
21.1 1128.36 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuAc,p@270--8a2D-NeuAc,p)--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
22.4 853.32 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuGc,p@270)--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
23.3 837.3 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
25.3 708.3 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--4a1D-Gal,p--3b1D-GalNAc,p$MONO,Und,-H,0,redEnd
25.3 652.72 [M -2H]2- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuGc,p@270$MONO,Und,-2H,0,redEnd
26.2 644.75 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p@270$MONO,Und,-H,0,redEnd
26.7 546.23 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
26.8 546.2 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--3b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
26.8 746.19 [M -2H]2- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p--3b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p$MONO,Und,-2H,0,redEnd
28.0 999.36 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--4b1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p@270$MONO,Und,-H,0,redEnd
28.0 999.36 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
29.5 505.2 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--4a1D-Gal,p$MONO,Und,-H,0,redEnd
29.7 925.36 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--3a2D-NeuAc,p@270--8a2D-NeuAc,p$MONO,Und,-H,0,redEnd
29.8 1202.43 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--4b1D-GalNAc,p--3b1D-Gal,p(--3a2D-NeuAc,p@270)--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
30.0 708.3 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--4b1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
34.9 650.21 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--3a2D-NeuGc,p@270$MONO,Und,-H,0,redEnd
35.8 634.32 [M -1H]1- redEnd--??1D-Glc,p--4b1D-Gal,p--3a2D-NeuAc,p@270$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column Hypercarb

Method

  • 73min gradient with 0,6ul/min run rate
  • 10mM ammonium bicarbonate
  • 60% ACN, 10mM ammonium bicarbonate

Mass Spec

Bruker Daltonik amaZon Speed ETD ETD

MIRAGE

See report

UniCarb-DR Creative Commons License

Supported by SIB ExPASy  |  JST / NBDCDICP