The Barramundi (Lates Calcarifer) mucus glycome display similar glycosylation in skin and gills and with large inter-individual variation

Investigator: Sara Linden

Submitter: Kristina Thomsson Hulthe

File Name: 2024_barramundi.gwp

GlycoPOST Entry: GPST000507

Retention Time Recorded Precursor Mass Structure
3.97 384.15 redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
7.74 837.3 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
8.13 472.17 redEnd--??1D-GalNAc,p--??2D-KDN,p$MONO,Und,-H,0,redEnd
9.19 464.11 redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
9.41 837.3 redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p--??2D-KDN,p$MONO,Und,-H,0,redEnd
9.45 425.18 redEnd--??1D-GalNAc,p--3a1D-GalNAc,p$MONO,Und,-H,0,redEnd
9.61 1040.38 redEnd--??1D-GalNAc,p--??1D-Gal,p}(--??1HexNAc,p--??1HexNAc,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
9.85 634.22 redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
9.99 587.23 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
9.99 513.19 redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
10.41 878.33 redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p}--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
10.5 722.24 redEnd--??1D-GalNAc,p--3a2D-KDN,p}--6a2D-KDN,p$MONO,Und,-H,0,redEnd
10.76 716.27 redEnd--??1HexNAc,p(--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
10.99 895.34 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
11.05 675.25 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
11.21 878.33 redEnd--??1D-GalNAc,p--3b1D-Gal,p}--??1HexNAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
11.31 884.29 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??2D-KDN,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
11.46 675.24 redEnd--??1D-GalNAc,p(--??1HexNAc,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
11.47 675.25 redEnd--??1D-GalNAc,p(--??1HexNAc,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
11.65 837.3 redEnd--??1D-GalNAc,p--??1HexNAc,p--??1D-Gal,p}--??2D-KDN,p$MONO,Und,-H,0,redEnd
11.74 790.31 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
12.3 1040.38 redEnd--??1D-GalNAc,p--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p}--??2D-KDN,p$MONO,Und,-H,0,redEnd
12.47 790.31 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
12.61 749.28 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
12.66 993.39 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
12.7 878.33 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.76 1081.4 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--??1HexNAc,p--??1HexNAc,p}--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.08 1301.5 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p}--??1HexNAc,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
13.42 675.24 redEnd--??1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.48 905.84 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p)--6b1D-GlcNAc,p(--??1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
13.84 1040.38 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--??2D-KDN,p$MONO,Und,-H,0,redEnd
13.98 1128.39 redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1HexNAc,p)--??2D-KDN,p}--??2D-KDN,p$MONO,Und,-H,0,redEnd
14.12 716.27 redEnd--??1HexNAc,p(--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.14 878.33 redEnd--??1D-GalNAc,p--??1HexNAc,p}(--??2D-NeuAc,p)--??1D-Gal,p$MONO,Und,-H,0,redEnd
14.23 853.33 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
14.85 1243.46 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p}--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.89 1108.9 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p}(--??1HexNAc,p--??1HexNAc,p)--??1HexNAc,p--??1HexNAc,p$MONO,Und,-2H,0,redEnd
15.15 897.33 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--6b1D-GlcNAc,p@360(--??1D-Gal,p(--??1HexNAc,p)--??2D-NeuAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
15.22 824.3 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p}--??2D-NeuAc,p$MONO,Und,-2H,0,redEnd
15.5 780.3 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1HexNAc,p$MONO,Und,-2H,0,redEnd
15.71 966.34 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.8 1312.0 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GlcNAc,p(--??1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p(--??1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--??1L-Fuc,p}--??1HexNAc,p--??1HexNAc,p$MONO,Und,-2H,0,redEnd
16.26 919.35 redEnd--??1D-GalNAc,p--??1HexNAc,p--??1HexNAc,p}--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.75 1040.38 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
17.03 911.34 redEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p)--6a1D-Man,p$MONO,Und,-H,0,redEnd
19.16 774.22 redEnd--??1D-GalNAc,p(--?b1D-Gal,p--??1S)--?a2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.24 1040.38 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.9 1446.54 redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p}(--??1HexNAc,p--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.4 1081.25 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
21.86 1331.47 redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
23.61 795.79 redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p}(--??1HexNAc,p--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-2H,0,redEnd
24.3 1110.37 freeEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-2H,0,freeEnd
31.8 1439.62 freeEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--??1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p)--6a1D-Man,p(--3b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-2H,0,freeEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Lates calcarifer
Tissue - Mucus(skin, gill, mouth, intestine)

Mass Spec

Manufacturer: Thermo Fisher

Model: Fusion orbitrap

Ion Mode: Negative

MIRAGE

See report

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