MIRAGE upload

redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd

Transient expression of PSGL-1 in CHO with different glycan profile

GlyTouCan Accession Number: Loading...

Peak	Intensity
160.7	0.1
165.96	0.4
176.92	0.3
177.38	0.1
178.97	101.5
179.69	0.3
179.92	0.1
181.06	0.1
183.94	1.9
185.76	0.2
186.16	0.2
192.22	0.1
195.95	0.2
202.01	2.1
204.02	48.9
204.95	0.2
206.92	0.1
207.96	0.4
208.32	0.1
209.99	0.1
219.04	0.1
221.02	4.0
222.06	15.8
226.0	0.1
226.96	0.1
235.24	0.1
238.25	0.1
247.13	0.1
253.97	0.1
254.89	0.1
255.95	0.1
257.12	0.2
262.87	13.6
267.14	0.1
270.1	0.2
275.33	0.1
278.63	0.1
279.8	0.2
280.25	0.3
280.91	2.7
285.98	0.5
299.17	3.0
304.01	1.7
305.01	0.1
306.01	0.1
307.13	0.2
315.78	0.2
316.8	0.2
317.17	0.9
321.79	0.1
324.15	0.2
328.03	0.1
329.09	0.1
333.37	0.1
335.64	0.2
345.9	0.3
347.13	1.8
347.97	0.2
354.37	0.1
356.13	0.2
359.07	1.2
364.09	56.2
365.26	1.0
366.31	0.1
376.05	0.1
376.41	0.1
377.27	0.2
378.23	0.4
382.11	0.1
385.3	0.1
389.36	0.7
407.11	40.0
420.09	0.1
425.05	0.4
425.32	0.8
426.03	0.1
427.1	0.2
427.45	0.2
437.19	2.9
439.89	0.1
467.34	0.4
468.09	0.3
471.37	0.1
475.02	0.1
479.17	42.1
485.3	1.1
488.95	0.3
490.62	0.3
491.26	0.2
496.72	0.1
497.29	0.2
527.15	25.8
527.99	0.1
542.95	0.3
545.18	10.5
550.78	0.1
551.04	0.1
553.15	0.1
557.35	0.1
557.87	0.1

There are 16 similar spectra:

Id	Structure	Score
5222		1.00
5695		1.00
3742		0.99
5547		0.98
3869		0.98
5195		0.97
4835		0.90
5935		0.88
5509		0.77
5015		0.76
6700		0.73
3743		0.65
6701		0.61
4231		0.60
5223		0.60
7700		0.52

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 586.24
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Cricetulus Griseus

Tissue - Cell Line, Transformed

HPLC

Column Custom made Hypercarb

Method

gradient with 10 ul/min run rate
10 mM Ammonium Biocarbonate
10 mM Ammonium Biocarbonate in 80% AcCN

Mass Spec

Thermo Fisher Scientific LTQ Orbitrap ESI

MIRAGE Structural data

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