MIRAGE upload

Recombinant mucin-type proteins carrying LacdiNAc on different O-glycan core chains fail to support H. pylori binding

GlyToucan Accession Number: G65285QO

Peak	Intensity
193.8	0.3
199.55	0.1
200.21	0.3
201.75	0.3
203.64	0.4
204.03	0.4
212.02	0.5
212.29	0.4
218.58	0.2
219.84	1.2
229.96	0.3
243.87	0.3
248.16	0.1
255.88	0.3
256.1	0.3
271.67	0.6
272.08	1.3
289.99	102.1
290.79	0.2
301.91	0.2
307.86	1.9
308.12	1.9
334.01	0.2
347.77	0.2
348.06	0.2
357.02	0.3
366.03	0.3
366.28	0.1
383.43	0.3
384.08	4.2
385.03	0.2
391.88	0.5
410.21	0.2
417.2	0.4
424.19	0.2
434.79	0.5
435.34	0.3
436.26	0.6
451.08	1.3
453.1	0.5
454.04	15.3
454.91	0.3
459.01	0.6
469.24	0.4
471.09	0.1
477.13	4.6
489.36	0.1
492.73	0.5
493.06	0.7
494.14	1.1
495.21	3.6
495.99	0.2
497.16	0.3
507.0	0.1
513.08	3.6
513.58	0.1
514.11	2.5
515.11	1.2
537.33	0.4
538.77	0.2
546.69	0.2
553.62	0.2
567.13	1.1
567.45	0.3
567.91	0.4
569.89	0.7
579.1	0.3
584.56	0.2
586.0	0.4
589.44	0.2
589.85	0.3
590.46	0.2
591.8	0.2
594.2	0.3
597.11	25.1
604.17	0.2
607.06	0.2
612.72	0.1
614.24	0.9
615.08	37.3
619.94	0.1
622.49	0.4
630.67	0.2
631.12	0.5
639.14	0.9
640.94	0.1
643.5	0.5
644.1	0.4
645.73	0.2
648.82	0.3
650.35	0.2
655.19	0.2
656.13	5.4
657.12	176.2
664.71	0.4
665.64	0.2
674.42	0.3
674.86	0.3
675.75	0.2
676.33	0.4

There are 18 similar spectra:

Id	Structure	Score
3971		0.88
5182		0.60
3492		0.54
5188		0.54
5422		0.54
5198		0.54
7620		0.54
4078		0.54
7681		0.53
7688		0.53
3746		0.53
4232		0.53
5224		0.53
4801		0.53
7693		0.52
7236		0.52
3972		0.51
3113		0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 674.25
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column Hypercarb

Method

Over 40 min gradient with 0.010 mL/min run rate
10 mM NH4HCO3
0-40% acetonitrile in 10 mM NH4HCO3

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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