Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
238.91 0.7
257.33 0.5
262.18 10.1

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd ^{2,4}A_{Gal} b1D-Gal,p/#acleavage_2_4--4?1HexNAc,p$MONO,Und,-H,0,redEnd
274.7 0.8
276.1 1.7
300.09 0.8
312.17 1.6
322.16 0.7

Annotations:

^{0,2}A_{Gal} b1D-Gal,p/#acleavage_0_2--4?1HexNAc,p$MONO,Und,-H,0,redEnd
324.02 1.3

Annotations:

^{2,4}X_{Gal}Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
330.31 2.8
335.27 0.9
338.07 0.9
344.13 1.2
346.14 1.0
347.14 4.8
348.56 1.2

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
359.48 1.4
364.36 3.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--4?1HexNAc,p$MONO,Und,-H,0,redEnd
365.35 4.6

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
366.17 2.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
370.26 1.8
371.12 3.3
377.02 0.6
381.77 0.7
382.09 3.1

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--4?1HexNAc,p$MONO,Und,-H,0,redEnd
382.96 0.6
383.23 0.7
389.1 8.6
389.35 5.9
391.18 0.7
405.1 0.6
406.46 0.7
407.2 270.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
408.23 2.0
425.23 79.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
437.26 12.5
438.43 0.8
441.34 0.6
445.24 0.7
448.95 1.4
456.31 1.4
460.39 2.4
465.22 0.7
471.11 1.1
476.23 0.6
479.3 20.1
480.32 0.9
481.25 1.7
485.21 1.0
485.4 0.6
495.37 0.8
509.37 2.0
509.79 1.2
521.14 1.1
527.28 29.0

Annotations:

^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd ^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--4?1HexNAc,p$MONO,Und,-H,0,redEnd
527.57 6.5
545.02 1.3
548.23 0.6
550.1 1.0
551.35 1.5
567.22 13.0
568.4 2.5
568.79 1.7
569.32 18.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
570.1 0.9
571.07 2.0
572.04 3.5
573.17 0.9
587.25 101.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
588.27 4.1
588.96 0.5
610.46 12.7
627.87 0.5
628.3 1.1
628.51 1.1
640.88 1.6
660.14 1.4
664.02 0.7
670.25 1.9
670.49 0.5
674.45 1.7
682.53 3.0
687.96 0.9
688.36 0.6
692.14 1.9
698.06 0.8
703.85 0.5
714.44 0.7
720.65 1.6
724.22 2.2
730.33 95.2
736.25 0.7
744.09 0.6
746.09 0.7
748.2 24.7
748.44 21.8
760.21 2.0
760.7 0.9
772.76 1.1
800.31 1.6

There are 14 similar spectra:

Id Structure Score

0.92

3755

0.90

5716

0.89

6724

0.89

3120

0.88

5518

0.88

6803

0.86

5684

0.76

7886

0.74

7998

0.71

7887

0.60

4851

0.58

0.58

5715

0.55

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 789.32
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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