Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
389.23 1.4

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
407.19 5.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
424.2 0.9

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.24 0.8

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
508.92 1.5
521.36 0.8
527.11 8.1

Annotations:

^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
527.49 3.9

Annotations:

^{0,4}X_{Gal}Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_4)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
545.24 1.5
551.1 12.6

Annotations:

B ?1D-Gal,p/#bcleavage--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.4 3.7
567.33 3.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd BY b1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.28 21.8

Annotations:

C ?1D-Gal,p/#ccleavage--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
587.23 23.8

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
625.3 1.9
637.04 1.4
637.3 1.4
641.08 0.8
653.25 1.0
665.13 0.9
671.22 0.9
677.45 0.8
683.45 0.9
685.52 1.2
689.31 10.0
695.25 1.5
707.17 3.7
707.47 2.1
712.17 1.1

Annotations:

^{2,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
713.34 17.1

Annotations:

B b1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
730.37 0.7

Annotations:

^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
731.38 2.2

Annotations:

C b1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
749.45 11.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
753.84 0.8
754.2 1.9

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
754.39 0.7
755.31 0.7
770.31 1.1

Annotations:

BY ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
772.28 7.6

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
772.59 2.2
773.32 11.7
787.27 1.4
790.49 4.6

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
812.6 0.7

Annotations:

^{0,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
814.49 0.7
815.38 2.9
844.43 1.0
873.81 0.8
874.44 1.8
892.19 3.6
892.55 1.8
897.98 0.9
909.17 0.8
910.29 2.9
910.59 0.8
916.4 16.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
916.91 0.7
934.29 2.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
934.66 1.1
936.18 0.7
952.31 19.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
952.84 1.0
957.48 0.9

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
975.4 0.8

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
975.95 1.2
976.47 7.8

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
998.24 0.7
1017.53 1.1
1018.39 1.3
1037.53 0.7
1047.49 0.9
1049.37 0.7
1059.23 0.8
1059.69 1.4
1061.11 1.8
1061.44 3.3
1077.52 1.4
1078.7 1.4
1079.38 44.4

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1089.41 1.9
1089.68 1.0
1097.35 8.6
1097.67 2.3
1098.11 0.8
1102.81 0.7
1103.48 1.8
1119.44 1.1
1121.4 127.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1130.06 0.7
1137.26 1.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
1139.39 86.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1151.53 1.0
1155.47 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
1181.35 3.3
1193.55 1.0
1202.73 0.7
1223.46 1.0
1241.4 112.4
1259.45 54.9
1271.02 1.4

There are 3 similar spectra:

Id Structure Score

5761

0.56

4821

0.50

0.50

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1300.51
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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