Structure

    redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
222.06 1.9

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
347.96 6.9
348.26 11.2

Annotations:

BY ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ ?1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
354.45 1.8
365.2 2.6

Annotations:

CY ?1D-Gal,p/#ccleavage--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd C ?1HexNAc,p/#ccleavage--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
365.91 13.4

Annotations:

CY ?1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
366.21 6.1
387.02 1.9

Annotations:

BZ ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
405.26 2.4

Annotations:

BY ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.3 2.3

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
466.23 5.5

Annotations:

C ?1D-Gal,p/#ccleavage--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-2H,0,redEnd
527.36 1.7
535.25 2.1
537.33 2.4
545.4 1.7
547.31 3.1

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd C a1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
547.93 2.5
551.26 53.6

Annotations:

B ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
552.21 11.9
568.18 1.9

Annotations:

YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-2H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd
568.94 8.4

Annotations:

C ?1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.71 3.6
577.25 29.3

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd
577.81 13.4
579.29 3.3
587.3 8.6

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.2 2.8
603.36 1.9
606.58 1.7
609.21 2.5
624.75 8.9
625.24 3.5
625.83 3.3
631.22 2.3
640.29 7.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
645.71 10.0
646.34 1.8
648.76 8.5

Annotations:

C ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
649.26 1.7
652.0 2.4
653.5 2.6
655.87 1.7
660.68 2.2
669.35 3.7
669.92 4.2

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-2H,0,redEnd ^{0,2}X_{HexNAc} redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
670.38 3.3

Annotations:

^{0,2}X_{HexNAc} redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
670.59 2.0
678.81 10.6

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd
679.26 11.6
685.46 2.3
687.24 5.7
689.34 5.5
698.46 2.0
701.44 3.3
706.98 1.8
708.26 7.8
713.36 15.7

Annotations:

CZ ?1D-Gal,p/#ccleavage--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd BY ?1D-Gal,p/#bcleavage--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd B ?1HexNAc,p/#bcleavage--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
714.4 3.7
715.22 11.7
716.42 11.5
717.14 2.2
721.79 50.0
722.26 49.0
724.59 1.9
772.39 7.7

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
773.31 53.2

Annotations:

^{2,4}A_{HexNAc} ?1HexNAc,p/#acleavage_2_4--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
774.33 6.0
790.41 24.8

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
791.44 7.8
815.19 167.3
816.34 20.0
833.22 6.4

Annotations:

^{0,2}A_{HexNAc} ?1HexNAc,p/#acleavage_0_2--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
833.93 3.8
834.44 2.1
868.33 2.1
892.3 12.1
892.72 3.1
916.44 7.1

Annotations:

B ?1D-Gal,p/#bcleavage--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
917.4 4.4
934.34 6.1

Annotations:

C ?1D-Gal,p/#ccleavage--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
934.82 2.0
935.61 2.5
952.34 257.5

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
953.32 73.3
960.42 2.2
975.42 3.1
1047.82 1.7
1078.37 3.1

Annotations:

CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd B a1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1078.57 1.8
1095.51 4.0
1119.2 2.3
1119.53 2.4

Annotations:

ZZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
1137.33 7.2

Annotations:

YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
1137.59 3.0
1138.35 5.5
1155.44 28.6

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
1156.56 13.9
1281.32 5.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--4?1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1281.61 1.9
1282.14 2.2

There are 5 similar spectra:

Id Structure Score

5484

0.80

5764

0.79

4826

0.67

5763

0.62

4827

0.55

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1503.59
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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