Structure

    redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3?1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
323.07 12.2
348.21 14.0

Annotations:

BY ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ ?1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
387.27 4.3

Annotations:

BZ ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
424.15 46.1

Annotations:

^{0,4}A_{GalNAc}Y a1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc}Y a1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
528.12 4.0

Annotations:

C a1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
544.24 6.0
551.23 126.6

Annotations:

B ?1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
552.39 15.0
561.01 3.9
567.2 6.4

Annotations:

BY b1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
568.54 6.0

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-2H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-2H,0,redEnd
577.42 8.3

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-2H,0,redEnd
584.39 6.4
602.47 23.6
609.0 5.1
612.24 6.9
635.35 4.2
648.96 3.7
668.93 4.2
681.26 8.8
682.26 7.4
683.25 4.3
713.31 10.0

Annotations:

B b1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
721.97 18.1
722.35 24.2

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
730.8 6.3
731.3 8.6

Annotations:

C b1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
742.76 3.7

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
751.78 133.5

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd BZ a1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
752.33 57.9
752.76 14.3
773.28 169.3

Annotations:

^{0,4}A_{GalNAc}Y a1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
774.25 46.4
775.27 7.5
791.28 7.3
793.1 3.8
799.3 7.3
814.3 13.1
814.82 5.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
820.35 22.9
820.91 16.8
821.38 4.8
822.16 3.7
835.2 5.4
838.46 4.1
853.38 17.5

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
853.85 11.2
854.23 4.8
872.34 6.4
872.88 6.1
880.27 5.2
881.32 3.8
889.29 4.2
890.26 7.5
892.42 13.0
892.66 4.9
893.28 8.7
896.41 72.6
896.85 50.5
897.37 22.8
898.51 3.8

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
899.33 6.7
952.4 70.1

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
953.37 25.0
1029.45 5.4
1047.45 3.9
1089.56 7.7
1119.46 4.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1121.43 23.2

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
1122.66 5.0
1137.22 7.1

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
1137.52 4.9
1138.39 6.9
1139.6 20.2

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
1140.37 4.0
1155.33 9.9

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
1241.35 50.8
1241.68 14.4
1242.37 23.7
1242.7 10.8
1243.53 5.3
1260.7 4.7
1283.41 4.2

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
1300.6 5.3
1301.46 385.1

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
1302.42 144.8
1303.5 20.9
1324.38 9.7

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1342.49 6.5

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1444.56 17.0
1445.44 5.3

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1446.4 4.6
1486.43 22.0

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1487.57 7.4
1504.73 40.9

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1505.52 48.6
1506.41 13.6
1527.72 5.6
1528.39 5.2
1631.6 5.6

There are 4 similar spectra:

Id Structure Score

5499

0.92

5497

0.83

5502

0.81

5503

0.59

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1852.72
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

Report errors in this entry

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