Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
262.3 2.3
323.05 8.5
348.0 2.9

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
348.3 2.2
406.32 3.0

Annotations:

^{0,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
408.24 2.3
424.26 9.5

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
549.19 3.4

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-2H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd BZ b1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.21 62.4

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
552.37 6.3
561.04 3.7
569.12 3.9
569.4 7.3

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-2H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.86 4.5
570.08 2.2
577.93 2.2
601.51 11.5
602.01 8.2
602.51 21.0
635.22 4.1
649.5 2.4
665.27 3.6
680.27 2.8
682.09 6.3
683.35 2.8
713.22 8.5

Annotations:

B b1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
722.8 4.5

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
731.15 3.1

Annotations:

C b1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
739.49 2.7
751.81 23.6

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd BZ ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
752.35 18.0
752.84 9.8
760.79 4.2
772.32 5.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
773.27 46.0

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
774.3 8.8
799.87 2.9
815.06 7.9
815.36 4.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
820.29 4.6
835.8 2.5
844.16 2.2
844.37 3.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-2H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
844.77 2.3
845.3 6.8
853.32 5.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-2H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-2H,0,redEnd
853.83 2.3
864.44 2.5
865.32 3.1
872.46 4.7
874.26 3.3
875.9 3.7

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
881.42 3.4
882.28 3.7
883.31 3.7
890.33 4.5
891.86 2.2
896.44 9.2
896.92 8.5
897.31 9.8
952.3 11.4
952.6 5.0
953.2 4.0
953.46 4.5
976.72 3.3

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
977.22 3.7
1061.36 4.0
1062.44 2.8

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
1103.08 2.8
1103.45 2.4
1121.47 134.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1122.53 41.1
1123.52 14.4
1139.32 39.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1140.55 4.9
1141.31 3.7
1155.43 3.3

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
1241.54 19.5
1242.12 2.5
1242.66 2.8
1243.46 2.4
1243.7 3.6
1259.7 2.8
1284.36 3.4
1301.45 205.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1302.47 125.6
1303.38 32.1
1303.69 11.6
1343.21 2.2
1439.58 3.0
1486.25 2.4
1486.69 3.9

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1487.48 4.4
1487.87 4.6
1504.54 12.1

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
1504.87 2.7
1505.42 6.4
1505.75 6.7
1506.71 2.5
1527.69 5.0

There are 3 similar spectra:

Id Structure Score

5497

0.87

5499

0.87

5501

0.81

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1852.72
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

Report errors in this entry

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