Structure

    redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Stress impairs skin barrier function and induces α2-3 linked N-acetylneuraminic acid and core 1 O-glycans on skin mucins in Atlantic salmon, Salmo salar

GlyTouCan Accession Number: Loading...

Peak Intensity
324.08 0.8
348.0 0.5

Annotations:

B ?1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
364.03 4.1

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd BY ?1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd BY b1D-Gal,p/#bcleavage--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.16 0.4
384.11 2.9

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
407.2 1.4
431.16 1.0
433.96 0.5
437.4 0.8
459.15 0.5
461.01 0.4
479.35 1.1
480.19 0.5
509.19 0.7
510.06 3.3

Annotations:

B ?1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
510.34 0.8
523.16 0.4
527.25 3.3
528.82 0.5
545.11 3.6
545.41 0.8
546.37 0.6
548.77 0.7
549.28 1.3

Annotations:

BZ b1D-Gal,p/#bcleavage--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.38 0.8
569.1 5.2

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
569.38 5.4
569.97 7.2
570.35 6.8
571.28 1.0
587.16 22.3

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
588.17 3.8
589.32 0.7
593.73 0.4
595.02 0.5
627.5 0.4
641.23 0.4
667.46 0.9
671.18 1.6
672.34 0.8
683.02 1.5
684.16 1.3
689.24 3.2
689.55 1.0
707.21 0.6
707.57 0.6
713.39 12.5

Annotations:

B b1D-Gal,p/#bcleavage--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
714.13 4.7
714.41 1.2
715.26 0.5
731.42 0.5

Annotations:

C b1D-Gal,p/#ccleavage--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
749.14 6.1

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
750.36 1.7
773.11 0.7
774.19 0.5
787.25 0.4
806.16 0.7
815.13 0.4
818.12 0.5
827.0 1.0
833.27 0.8
834.22 0.4
838.11 0.8
843.23 0.4
859.75 0.7
874.16 1.4
874.53 0.8
875.16 5.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
875.46 2.7
876.12 1.6
876.46 0.7
892.41 2.0
900.37 0.4
916.32 0.4
918.37 3.0
919.15 2.1
919.6 0.4
934.05 0.5

Annotations:

Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
936.34 2.8
948.28 3.1
949.42 1.2
967.19 1.1
978.25 0.7
978.56 0.6
989.87 0.5
990.3 1.2
991.42 1.0
996.31 4.6
997.27 0.9
1000.31 0.5
1004.43 0.7
1005.42 0.4
1014.28 1.0
1038.31 62.8
1039.26 23.3
1054.11 0.7
1056.29 46.0
1057.33 15.3
1099.25 9.6
1099.62 2.8

There are 5 similar spectra:

Id Structure Score

0.99

4887

0.99

5745

0.69

5450

0.64

3983

0.53

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1097.43
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Mucus

HPLC

Column Hypercarb

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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