Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
347.26 0.9
347.99 1.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
358.25 1.2
364.24 1.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd BY ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.22 1.6
365.43 1.0
365.87 1.0

Annotations:

CY ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
383.25 1.0
384.35 1.5

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
389.18 5.7
407.19 35.5
408.27 0.8

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
424.18 1.7

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
425.45 1.2
434.19 1.4
450.22 0.9
461.39 0.8
467.21 2.3
485.21 0.9
503.28 2.7
509.11 4.8

Annotations:

^{2,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
509.33 2.6
510.17 10.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
521.32 1.2
526.72 1.6
527.3 13.4

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
545.26 2.2
551.07 0.8

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.22 45.7

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.19 35.7

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
587.28 50.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.16 1.8
612.1 3.8
655.11 0.8
664.09 0.9
675.12 0.8
677.22 0.8
688.89 1.6
689.43 6.4
695.36 0.8
707.26 2.6
711.11 1.1
712.24 2.9
712.54 1.0
713.3 18.7

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
731.25 0.9

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
748.28 6.0
749.33 16.8

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
749.69 3.1
750.35 3.7
754.56 1.7

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
755.45 1.6
771.25 1.1
772.33 1.2

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
772.69 1.0
772.98 1.2
773.33 9.2
774.36 1.1
798.29 0.9
806.2 1.0
815.28 1.8
822.76 0.8
826.49 1.3
827.38 1.3
833.29 1.3
840.44 1.1
844.48 1.9
858.35 9.4
873.91 0.8
874.38 1.5
874.7 1.6
875.31 3.6
876.33 59.2
881.92 0.8
894.39 9.7
913.94 1.4
916.32 1.7
916.51 1.5
918.33 148.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
919.05 1.3
919.41 1.1
934.4 1.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
936.36 79.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
937.46 1.6
948.44 1.7
952.51 2.8

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
953.27 1.1
978.42 1.6
990.53 1.8
996.32 2.7
1014.52 0.9
1036.13 1.2
1038.38 126.6
1039.44 8.8
1049.56 0.8
1051.28 0.9
1056.39 70.3
1057.43 5.5
1059.34 0.8
1073.44 1.0

There are 9 similar spectra:

Id Structure Score

5745

0.94

0.90

4814

0.90

3983

0.79

5811

0.70

4887

0.67

0.67

5746

0.64

5599

0.52

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1097.43
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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