Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-2H,0,redEnd

Sulfation of O-glycans on mucin-type proteins from serous ovarian epithelial tumors

GlyTouCan Accession Number: Loading...

Peak Intensity
167.88 0.3

Annotations:

^{1,5}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_1_5--4b1D-Gal,p$MONO,Und,-2H,0,redEnd
170.04 0.5

Annotations:

^{1,3}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_1_3--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S$MONO,Und,-2H,0,redEnd
184.05 0.5

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
212.32 0.6

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-2H,0,redEnd
221.07 0.3

Annotations:

^{2,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_2_4--4b1D-Gal,p$MONO,Und,-H,0,redEnd
221.45 0.6

Annotations:

^{2,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S/#lcleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_2_4--4b1D-Gal,p$MONO,Und,-H,0,redEnd
235.3 0.6

Annotations:

^{3,5}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_3_5--4b1D-Gal,p$MONO,Und,-H,0,redEnd
236.97 0.4

Annotations:

^{2,5}A_{Gal} b1D-Gal,p/#acleavage_2_5(--3a2D-NeuAc,p)--6?1S$MONO,Und,-2H,0,redEnd
253.96 0.6
256.13 0.4
260.84 0.3
269.83 0.5
270.45 0.3
276.83 0.5
278.82 0.3
284.94 0.9
290.08 10.0

Annotations:

B b1D-Gal,p/#bcleavage--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.08 1.2

Annotations:

^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S/#lcleavage$MONO,Und,-2H,0,redEnd
304.77 0.6
321.07 0.4
346.94 0.6
360.65 0.5
368.21 0.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-2H,0,redEnd
369.06 0.4
377.53 0.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-2H,0,redEnd
384.88 0.4
393.14 0.3
395.0 0.3
412.85 0.5
413.84 0.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-2H,0,redEnd
421.72 0.6
423.77 0.4

Annotations:

^{1,5}A_{Gal} b1D-Gal,p/#acleavage_1_5(--3a2D-NeuAc,p)--6?1S/#lcleavage$MONO,Und,-H,0,redEnd ^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
426.97 0.4

Annotations:

^{0,2}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_2(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-2H,0,redEnd
432.89 0.3
443.0 0.3
449.04 1.2
449.55 1.2
455.02 0.6

Annotations:

^{1,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_1_5(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-2H,0,redEnd
461.08 0.9
463.04 0.5
467.02 0.4
467.64 0.4
469.45 0.4

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-2H,0,redEnd
471.51 0.7
472.35 0.6
473.53 0.3
478.5 0.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-2H,0,redEnd
478.79 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-2H,0,redEnd
479.8 0.9
480.17 0.6
486.74 0.9
487.06 0.3
489.24 0.6
492.72 0.4
494.98 0.5
497.02 1.8
499.85 0.3

Annotations:

^{2,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_5(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-2H,0,redEnd
501.13 0.6
502.85 0.4
504.99 0.3
505.43 0.4
505.78 0.6
506.4 0.9
514.08 1.2
515.11 1.6
515.46 0.5
516.31 0.9
522.69 0.3
522.96 0.9
523.19 0.6
524.06 0.5
525.85 0.5
528.47 0.4
528.77 1.0
529.24 2.5
529.99 0.4
531.99 1.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S$MONO,Und,-H,0,redEnd
532.72 0.8
569.13 1.3

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S/#lcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#zcleavage)--6?1S/#lcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
587.11 0.5

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S/#lcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
649.2 0.3

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#zcleavage)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
675.29 1.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S/#lcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
676.21 0.3
693.42 1.1
694.35 0.5
738.33 0.3
745.8 0.3
746.13 0.4
810.95 0.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#zcleavage)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
811.22 1.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#zcleavage)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
827.26 0.3
829.24 89.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p/#ycleavage)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
830.28 30.1

Annotations:

^{1,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_1_5(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S/#lcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
831.02 1.0
831.37 0.4
897.24 0.4
899.29 1.5
899.49 0.9
900.27 0.7
1017.26 0.5

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd

There are 3 similar spectra:

Id Structure Score

1.00

6687

0.99

0.99

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1119.34
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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