Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p)--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-2H,0,redEnd

Rainbow trout gastrointestinal mucus, mucin production, mucin glycosylation and response to lipopolysaccharide

GlyTouCan Accession Number: Loading...

Peak Intensity
218.06 4.9
222.26 4.5

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
269.18 3.4
295.95 3.1
306.15 151.9

Annotations:

B a2D-NeuGc,p/#bcleavage--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
307.2 5.9
315.2 3.7

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-2H,0,redEnd
324.39 5.6

Annotations:

C a2D-NeuGc,p/#ccleavage--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
336.6 6.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p/#zcleavage$MONO,Und,-2H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p/#ycleavage$MONO,Und,-2H,0,redEnd
348.26 10.8

Annotations:

B b1D-Gal,p/#bcleavage--??1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p/#zcleavage$MONO,Und,-H,0,redEnd
378.07 4.1
384.2 20.3

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
406.06 3.7
422.88 3.0
461.21 8.2
463.41 3.1
477.83 4.3
489.71 5.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-2H,0,redEnd
490.3 6.2
495.11 3.1
498.72 18.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-2H,0,redEnd
499.28 6.6
511.44 6.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
515.55 4.8
521.46 3.3
532.07 3.8
537.38 3.9
537.86 4.9
543.35 11.8

Annotations:

^{0,2}A_{NeuGc} a2D-NeuGc,p/#acleavage_0_2--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
545.49 12.4
554.84 11.3
555.23 6.7
555.45 13.4
555.65 4.8
559.19 7.0
565.03 4.8
569.39 10.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#ycleavage)--6a2D-NeuGc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#zcleavage)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
582.38 7.5
587.21 3.6

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#ycleavage)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
589.56 4.1
590.97 3.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#zcleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-2H,0,redEnd
592.04 10.6
594.98 4.2
597.25 3.2
598.24 4.4
600.79 10.0
605.03 7.3
605.24 4.6
606.02 3.5
611.09 21.1
612.29 11.1
612.6 4.1
613.26 124.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
614.24 30.5
615.22 7.4
617.58 8.6
619.56 3.8
621.22 4.4
625.36 3.3
626.06 7.1
626.93 3.8
627.35 11.1
631.22 6.7

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
638.31 4.6
644.06 18.5
691.42 12.2

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
692.25 3.8
693.23 3.7
699.54 3.6
733.53 6.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
734.29 4.2
734.66 3.7
735.49 3.8
761.52 4.6
800.09 4.8
800.46 7.3
804.53 4.7
818.6 13.4

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
928.15 5.2
946.43 3.9
962.54 4.5
980.38 8.6
980.62 11.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p--8a2D-NeuGc,p$MONO,Und,-H,0,redEnd
981.21 7.1
981.59 7.2
982.14 7.8
1004.72 3.5
1022.23 14.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p)--6a2D-NeuGc,p--8a2D-NeuGc,p/#zcleavage$MONO,Und,-H,0,redEnd
1022.84 11.4
1023.13 3.1
1023.44 20.8
1023.78 6.2
1040.45 390.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p)--6a2D-NeuGc,p--8a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
1041.65 90.1
1042.59 38.4
1042.98 4.0
1103.45 3.0
1253.58 11.2
1254.14 5.1
1254.62 3.0

There are 4 similar spectra:

Id Structure Score

5218

0.75

3523

0.67

4220

0.66

8055

0.60

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1346.48
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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