Structure

    redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
149.95 1.4
152.12 0.8
153.88 2.6
155.65 0.3
156.03 0.3
160.09 0.3
161.23 0.2
161.98 0.5
164.07 0.2
165.94 0.7
167.99 2.1
169.97 12.5
170.9 0.3
172.07 0.4
175.82 0.4
177.99 0.2
180.01 0.6
182.01 0.3
186.12 0.3
189.9 0.2
192.18 0.2
194.01 1.3
194.41 0.2
195.16 0.3
196.17 0.5
198.1 0.4
200.04 0.4
201.07 0.4
202.14 0.8
203.76 1.1

Annotations:

Z redEnd--??1D-GalNAc,p--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
204.04 0.9

Annotations:

Z redEnd--??1D-GalNAc,p--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
208.21 0.4
208.95 0.2
211.07 0.2
211.95 0.4
212.18 0.5
213.93 0.3
218.05 0.3
219.03 0.2
220.18 1.3
220.84 0.3
222.1 4.3

Annotations:

Y redEnd--??1D-GalNAc,p--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
223.22 0.7
227.25 0.2
227.57 0.2
228.84 0.3
230.07 1.0
231.71 0.2
244.79 0.2
246.23 0.9
247.02 0.4
255.98 0.2
264.74 0.2
272.25 1.8
274.12 1.8
275.02 0.3
276.12 9.8
277.17 0.9
283.47 0.2
288.08 0.6
290.11 353.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.14 40.6
292.07 10.1
293.08 1.1
295.36 0.5
297.24 0.4
299.15 0.3
302.21 0.6
306.16 0.6
307.3 0.3

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
308.03 1.2

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
308.8 0.3

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
309.09 0.9

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
319.32 0.3
323.16 0.5
334.11 3.9
335.26 1.5
338.84 0.2
345.98 0.4
351.11 0.6
357.31 0.3
358.21 0.7
365.03 0.3

Annotations:

^{3,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_3_5--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
373.36 0.2
375.16 1.1
375.42 0.3
390.2 0.4
405.42 0.7
408.31 0.2
408.89 0.2
409.15 0.4

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
409.45 0.3

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
409.8 0.5

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
410.31 0.2

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
411.26 2.5
412.36 0.3
415.05 0.3
417.59 0.4
424.04 0.2
427.14 0.4

There are 19 similar spectra:

Id Structure Score

5540

1.00

3737

1.00

7389

1.00

7418

1.00

7162

1.00

7178

1.00

3866

1.00

5411

0.99

4798

0.99

5689

0.99

6792

0.99

5506

0.99

4076

0.99

6445

0.98

6506

0.98

6514

0.98

6522

0.98

6580

0.98

3969

0.78

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 512.20
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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