Structure

    redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

500.0100015002000m/z0.0204060801.0e+2Abundance
Peak Intensity
347.13 1.3
363.99 1.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd BY ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
364.37 1.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd BY ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
372.88 0.9
407.21 1.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.29 1.8

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
477.3 0.9
509.66 1.0

Annotations:

B ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
527.42 5.6

Annotations:

C ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
551.39 2.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.4 6.8

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.46 2.9
587.37 38.9

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.49 2.1
597.33 2.1
616.35 1.2
662.26 1.1
664.32 4.2
664.61 2.1
665.43 1.8
675.45 1.9
675.73 1.3
682.34 2.9
682.66 3.7
688.52 1.5
707.44 1.4
716.42 3.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
717.42 2.2
718.23 1.4
724.04 1.0
724.49 4.4
725.46 2.0
754.36 1.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
772.4 2.3

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
773.47 1.4
790.41 2.3

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
791.42 1.0
800.43 2.6
801.34 2.6
801.59 1.3
818.54 6.4
819.62 3.4
823.38 1.1
842.33 1.1
843.18 1.0
858.57 1.5
860.52 10.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.83 2.6

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.45 4.0
861.67 1.8
874.39 1.4
876.35 1.3
877.23 0.9
878.35 21.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
878.69 12.1

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.39 6.3
879.65 1.7
918.59 3.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
918.89 1.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
919.26 1.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
919.66 1.0
936.42 27.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
937.33 8.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
937.59 3.9
955.36 1.2
967.45 0.9
968.38 0.9
988.4 2.8
988.65 4.5
1006.15 8.0
1006.7 17.5
1007.67 9.7
1009.58 0.9
1045.58 1.7
1063.68 8.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
1064.81 2.5
1081.6 15.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
1082.58 8.6
1083.4 1.9
1084.59 1.7
1090.52 0.9
1106.87 1.4
1110.58 1.5
1119.69 1.8
1147.44 1.3
1149.55 29.0
1150.52 3.9
1150.83 5.0
1165.47 4.2
1165.73 1.3
1167.75 91.2
1168.59 75.4
1185.48 1.8
1186.44 1.0
1189.49 1.0
1189.81 0.9
1191.27 2.6
1191.55 2.2
1192.84 1.0
1853.79 0.9

There are 2 similar spectra:

Id Structure Score

6819

0.78

5835

0.66

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1226.47
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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