N-glycans and O-glycans released from purified mammalian glycoprotein standards and proteins from cell lysate

Ashwood C, Manuscript

Retention Time Recorded Precursor Mass Structure
4.82 675.27 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
8.19 675.3 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
42.59 1111.53 [M -2H]2- redEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--6a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--6a2D-NeuAc,p$MONO,Und,-2H,0,redEnd
43.18 965.97 [M -2H]2- redEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--6a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p$MONO,Und,-2H,0,redEnd
46.25 812.38 [M -2H]2- redEnd--?b1D-GlcNAc,p(--6a1L-Fuc,p)--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-2H,0,redEnd
47.5 812.37 [M -2H]2- redEnd--?b1D-GlcNAc,p(--6a1L-Fuc,p)--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--??1D-Gal,p)--6a1D-Man,p--2b1D-GlcNAc,p$MONO,Und,-2H,0,redEnd
49.56 1111.58 [M -2H]2- redEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--3a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--6a2D-NeuAc,p$MONO,Und,-2H,0,redEnd
50.05 1038.92 [M -2H]2- redEnd--?b1D-GlcNAc,p(--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--6a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p)--6a1L-Fuc,p$MONO,Und,-2H,0,redEnd
50.53 965.97 [M -2H]2- redEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--3a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p$MONO,Und,-2H,0,redEnd
55.56 1111.54 [M -2H]2- redEnd--?b1D-GlcNAc,p--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--3a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-2H,0,redEnd
57.48 1038.94 [M -2H]2- redEnd--?b1D-GlcNAc,p(--4b1D-GlcNAc,p--4b1D-Man,p(--3a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p--3a2D-NeuAc,p)--6a1D-Man,p--2b1D-GlcNAc,p--?b1D-Gal,p)--6a1L-Fuc,p$MONO,Und,-2H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Mus Musculus
Tissue - Cells, Cultured
Cell line - J774A.1 cell

HPLC

Column Hypercarb

Method

  • gradient with 4ul per min run rate
  • 10mM Ammonium Bicarbonate in ultra-pure H2O
  • 10mM ammoinium bicarbonate in 45% (v/v) acetonitrile and 55% (v/v) ultra-pure H2O

Mass Spec

Thermo Fisher Scientific LTQ Velos QTOF

MIRAGE

See report

UniCarb-DR Creative Commons License

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