Structure

    redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd

N-glycans and O-glycans released from purified mammalian glycoprotein standards and proteins from cell lysate

GlyTouCan Accession Number: Loading...

200.0300.0400.0500.0600.0m/z0.01.0e+32.0e+33.0e+3Abundance
Peak Intensity
193.9999996 1.4791195393
204.0534857 35.7471365929

Annotations:

Z redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
214.0799991 1.0670747757
219.9641326 4.4255102873
222.1316989 18.8918031454

Annotations:

Y redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
224.0985248 2.5972867012
236.0799986 1.0127235651
246.0998039 3.1412795782
252.2187576 2.565038681
268.1599979 1.0255377293
272.0726292 48.1244134903
276.1334839 9.6382516623

Annotations:

^{0,3}X_{Gal}Z redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#xcleavage_0_3--3a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ^{0,3}X_{GalNAc}Z redEnd--?a1D-GalNAc,p/#xcleavage_0_3--3b1D-Gal,p--3a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
286.4954506 3.2900623083
290.0551799 3127.1339873075

Annotations:

B b1D-Gal,p/#bcleavage--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.6138961 4.8654913902
292.120353 3.9130655527

Annotations:

^{1,4}A_{GalNAc}Y a1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p--3a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{3,5}X_{Gal}Z redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#xcleavage_3_5--3a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ^{3,5}X_{GalNAc}Z redEnd--?a1D-GalNAc,p/#xcleavage_3_5--3b1D-Gal,p--3a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
324.1309696 9.4173907042

Annotations:

^{0,4}X_{Gal}Y redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#xcleavage_0_4--3a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{1,3}X_{Gal} redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#xcleavage_1_3$MONO,Und,-H,0,redEnd ^{2,4}X_{Gal} redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd ^{0,4}X_{GalNAc}Y redEnd--?a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--3a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
366.1594749 3.7027109861

Annotations:

Z redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
370.1831492 2.6212656498
374.2399955 1.0867916346
376.1999955 1.0641328096
380.1495927 32.0220532417

Annotations:

C^{0,3}X_{Gal} a1D-GalNAc,p/#ccleavage--3b1D-Gal,p/#xcleavage_0_3--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd ^{1,4}A_{Gal} b1D-Gal,p/#acleavage_1_4--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
384.1799168 126.190071702

Annotations:

Y redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
386.0799953 1.4377740622
406.2156254 3.1956322193
408.20302 5.4462759495

Annotations:

B^{1,3}X_{NeuAc} a1D-GalNAc,p/#bcleavage--3b1D-Gal,p--3a2D-NeuAc,p/#xcleavage_1_3$MONO,Und,-H,0,redEnd
416.1905482 6.8547407389
434.1581007 5.0964562893
454.1864897 9.5408360958

Annotations:

^{0,2}X_{NeuAc} redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
471.2200698 14.2165745497
477.2413535 6.1253228188
507.3599926 1.1659764051
614.2718793 5.2143080235
615.3641954 6.1515909433

Annotations:

^{0,4}X_{Gal} redEnd--?a1D-GalNAc,p--3b1D-Gal,p/#xcleavage_0_4--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd ^{0,4}X_{GalNAc} redEnd--?a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
631.3595469 54.1512237787

Annotations:

^{1,3}X_{NeuAc} redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p/#xcleavage_1_3$MONO,Und,-H,0,redEnd
639.3164104 6.4479665756

There are 80 similar spectra:

Id Structure Score

3493

1.00

7237

1.00

4079

1.00

1.00

6584

1.00

5423

1.00

6708

1.00

6798

1.00

1.00

1.00

7649

1.00

7665

1.00

5701

1.00

7621

1.00

5511

1.00

4802

1.00

1.00

4233

1.00

5225

1.00

5198

1.00

5389

1.00

1.00

7165

1.00

3747

1.00

3872

0.99

0.99

7682

0.99

7689

0.99

5551

0.99

4203

0.99

7141

0.99

0.99

7694

0.99

7181

0.99

7983

0.99

5188

0.99

7984

0.99

0.99

0.99

7392

0.98

7421

0.98

3972

0.98

5700

0.97

3636

0.96

5182

0.94

5550

0.93

0.93

6707

0.92

4232

0.92

5224

0.92

7236

0.91

4078

0.91

3492

0.91

3746

0.91

4202

0.90

7620

0.90

6797

0.89

5422

0.89

7180

0.89

7391

0.89

7420

0.89

0.89

6583

0.89

4801

0.89

0.89

5510

0.89

3871

0.88

3113

0.88

0.88

7681

0.88

7688

0.88

7982

0.88

7140

0.88

0.87

7693

0.87

7950

0.87

7164

0.86

3971

0.74

7880

0.68

5771

0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 674.25
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Mus Musculus
Tissue - Cells, Cultured

HPLC

Column Hypercarb

Method

  • gradient with 4ul per min run rate
  • 10mM Ammonium Bicarbonate in ultra-pure H2O
  • 10mM ammoinium bicarbonate in 45% (v/v) acetonitrile and 55% (v/v) ultra-pure H2O

Mass Spec

Thermo Fisher Scientific LTQ Velos QTOF

MIRAGE Structural data

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