Brachyspira hyodysenteriae Infection Regulates Mucin Glycosylation Synthesis Inducing an Increased Expression of Core-2 O-Glycans in Porcine Colon.

Venkatakrishnan V, Quintana-Hayashi MP, Mahu M, Haesebrouck F, Pasmans F and Lindén SK , Manuscript

Retention Time Recorded Precursor Mass Structure
15.6 895.3 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
16.5 667.3 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1S$MONO,Und,-H,0,redEnd
18.3 749.3 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
21.2 878.3 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
22.85 895.3 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
23.96 530.2 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--2a1L-Fuc,p$MONO,Und,-H,0,redEnd
27.9 895.3 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--2a1L-Fuc,p)--6b1D-GlcNAc,p--3/4b1D-Gal,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Sus Scrofa
Tissue - Intestine, Large

HPLC

Column Custom made Hypercarb

Method

  • 78 minutes gradient with 250 nL/min run rate
  • 10mM ammonium bicarbonate
  • 10mM ammonium bicarbonate in 80% Acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

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