Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--??1S$MONO,Und,-H,0,redEnd

Core-2 O-glycans are required for galectin-3 interaction with the synovial glycoprotein lubricin

GlyTouCan Accession Number: Loading...

Peak Intensity
138.08 2.0
138.66 0.7
139.2 0.0

Annotations:

^{2,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_5)--??1S/#lcleavage$MONO,Und,-2H,0,redEnd
139.77 0.0

Annotations:

^{2,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_5)--??1S/#lcleavage$MONO,Und,-2H,0,redEnd
140.28 0.0
140.83 0.0
141.36 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--??1S$MONO,Und,-2H,0,redEnd
141.9 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--??1S$MONO,Und,-2H,0,redEnd
142.44 0.0
142.97 0.0
143.52 0.0
156.03 1.4
156.6 0.0
157.12 0.0
157.69 0.0
158.2 0.0
158.74 0.0
159.28 0.0
159.81 0.0
160.36 0.0
160.87 0.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p$MONO,Und,-H,0,redEnd
166.96 0.8
167.52 0.0
168.03 0.0
168.58 0.0
169.11 0.0
169.65 0.0
170.19 0.0
170.72 0.0
171.27 0.0

Annotations:

^{0,2}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_2)--??1S$MONO,Und,-2H,0,redEnd
171.79 0.0

Annotations:

^{0,2}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_2)--??1S$MONO,Und,-2H,0,redEnd
176.87 0.8
177.44 0.0
177.95 0.0
178.52 0.0
179.03 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p$MONO,Und,-H,0,redEnd ^{2,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_5)--??1S$MONO,Und,-2H,0,redEnd
179.58 0.0

Annotations:

^{2,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_5)--??1S$MONO,Und,-2H,0,redEnd
180.05 1.8
180.61 0.0
181.14 0.0
181.69 0.0
182.21 0.0
182.77 0.0
183.29 0.0
183.85 0.0
184.37 0.0
184.93 0.0
185.27 1.8
185.83 0.0
186.36 0.0

Annotations:

^{0,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_3)--??1S$MONO,Und,-2H,0,redEnd ^{1,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_4)--??1S$MONO,Und,-2H,0,redEnd
186.9 0.0

Annotations:

^{0,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_3)--??1S$MONO,Und,-2H,0,redEnd ^{1,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_4)--??1S$MONO,Und,-2H,0,redEnd
187.44 0.0
187.97 0.0
188.52 0.0
189.05 0.0
189.61 0.0
190.12 0.0
194.04 1.0

Annotations:

^{3,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_3_5)--??1S$MONO,Und,-2H,0,redEnd
194.62 0.0

Annotations:

^{3,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_3_5)--??1S$MONO,Und,-2H,0,redEnd
194.94 0.0

Annotations:

^{3,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_3_5)--??1S$MONO,Und,-2H,0,redEnd
195.19 0.0
195.7 0.0
196.01 0.0
196.27 0.0
196.78 0.0
197.08 0.0
197.34 0.0
197.91 1.1
198.46 0.0
199.02 0.0
199.54 0.0
200.1 0.0
200.62 0.0
201.19 0.0

Annotations:

^{0,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_4)--??1S$MONO,Und,-2H,0,redEnd ^{1,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_3)--??1S$MONO,Und,-2H,0,redEnd ^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--??1S$MONO,Und,-2H,0,redEnd
201.7 0.0

Annotations:

^{0,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_4)--??1S$MONO,Und,-2H,0,redEnd ^{1,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_3)--??1S$MONO,Und,-2H,0,redEnd ^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--??1S$MONO,Und,-2H,0,redEnd
202.25 0.0
202.89 2.2
203.95 1.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
204.21 1.8

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
204.78 0.0
205.3 0.0
205.86 0.0
206.38 0.0
207.23 4.0
207.78 0.0
208.34 0.0
208.86 0.0
209.4 0.0
209.94 0.0
210.47 0.0
211.03 0.0
211.55 0.0
212.1 0.0
213.06 1.3
213.61 0.0
214.15 0.0
214.69 0.0
215.22 0.0
215.77 0.0
216.3 0.0
216.86 0.0
217.37 0.0
217.94 0.0
221.12 0.9
221.69 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
222.2 0.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
222.77 0.0
223.09 0.8
223.92 0.7
224.47 0.0
225.02 0.0
225.54 0.0
226.11 0.0
226.62 0.0
227.19 0.0
227.7 0.0
228.26 0.0
228.78 0.0
239.95 1.3
240.91 22.3
241.33 0.5
241.83 1.2
242.12 0.8
242.65 0.0
243.19 0.0
243.72 0.0
244.28 0.0
244.79 0.0
245.07 0.0
245.36 0.0
245.87 0.0
246.14 0.0
246.44 0.0
246.95 0.0
252.78 0.6
253.09 2.4
253.63 0.0
254.19 0.0
254.71 0.0
255.27 0.0
255.79 0.0
256.35 0.0
256.87 0.0
257.42 0.0
257.94 0.0
259.03 4.2
259.77 0.0
260.28 0.0
261.14 0.8
261.7 0.0
262.23 0.0
262.78 0.0
263.3 0.0
263.86 0.0

Annotations:

^{0,2}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_2)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
264.38 0.0

Annotations:

^{0,2}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_2)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
264.94 0.0
265.46 0.0
272.07 1.1
272.63 0.0
273.19 0.0
273.7 0.0
274.27 0.0
274.78 0.0
275.34 0.0
275.86 0.0
276.4 0.0
276.94 0.0
283.96 23.8

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--??1S$MONO,Und,-H,0,redEnd
284.49 1.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--??1S$MONO,Und,-H,0,redEnd
284.97 0.5
285.53 0.0
286.06 0.0
286.61 0.0
287.13 0.0
287.69 0.0
288.21 0.0
288.69 1.0
289.26 0.0
289.78 0.0
290.32 0.0
290.86 0.0
291.39 0.0
291.94 0.0
292.47 0.0
293.02 0.0
293.63 0.7

Annotations:

^{0,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_3)--??1S/#lcleavage$MONO,Und,-H,0,redEnd ^{1,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_4)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
294.19 0.0

Annotations:

^{0,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_3)--??1S/#lcleavage$MONO,Und,-H,0,redEnd ^{1,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_4)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
294.71 0.0
295.02 1.0
295.59 0.0
296.11 0.0
296.65 0.0
297.19 0.0
297.72 0.0
298.28 0.0
298.8 0.0
299.36 0.0
299.87 0.0
302.05 40.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--??1S$MONO,Und,-H,0,redEnd
302.49 3.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--??1S$MONO,Und,-H,0,redEnd
302.91 8.0
303.28 4.0
304.09 1.8
304.63 0.0
304.96 0.0
305.19 0.0
305.71 0.0
306.04 0.0
306.27 0.0
306.79 0.0
307.11 0.0
307.35 0.0
307.87 0.0
308.42 0.0
308.94 0.0
310.05 0.8

Annotations:

^{3,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_3_5)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
310.61 0.0

Annotations:

^{3,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_3_5)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
311.13 0.0
311.69 0.0
312.21 0.0
312.77 0.0
313.29 0.0
313.85 0.0
314.37 0.0
315.14 0.8
315.71 0.0
316.27 3.9
316.86 0.0
317.37 0.0
317.94 0.0
318.45 0.0
319.01 0.0
319.53 0.0
320.07 0.0
320.61 0.0
321.14 0.0
322.04 2.5
322.61 0.0
323.13 0.0
323.61 1.4
324.19 0.0

Annotations:

^{0,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_4)--??1S/#lcleavage$MONO,Und,-H,0,redEnd ^{1,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_3)--??1S/#lcleavage$MONO,Und,-H,0,redEnd ^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
324.7 0.0
325.27 0.0
325.78 0.0
326.33 0.0
326.86 0.0
327.4 0.0
327.94 0.0
331.91 3.4
332.46 0.0
333.02 0.0
333.54 0.0
334.1 0.0
334.62 0.0
335.32 1.9
335.87 0.0
336.44 0.0
336.95 0.0
337.5 0.0
338.03 0.0
338.57 0.0
339.11 0.0
339.64 0.0
340.31 1.2
340.86 0.0
341.39 0.0
341.94 0.0
342.47 0.0
343.07 3.4
343.96 4.3

Annotations:

^{0,2}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_2)--??1S$MONO,Und,-H,0,redEnd
344.77 2.3
345.15 0.5
345.7 0.0
346.24 0.0
346.78 0.0
347.31 0.0
347.86 0.0
348.38 0.0
348.94 0.0
349.46 0.0
361.09 2.2
361.67 0.0
362.19 0.0
362.75 0.0
363.27 0.0
363.79 0.0
364.34 0.0
364.87 0.0
365.46 0.6
366.04 2.7
366.27 1.3
366.61 0.0
366.86 0.0
367.37 0.0
367.69 0.0
367.94 0.0
368.45 0.0
368.77 0.0
369.02 0.0
369.53 0.0
369.83 0.0
370.1 0.0
370.61 0.0
373.13 0.8
373.67 0.0

Annotations:

^{0,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_3)--??1S$MONO,Und,-H,0,redEnd ^{1,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_4)--??1S$MONO,Und,-H,0,redEnd
374.2 0.0

Annotations:

^{0,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_3)--??1S$MONO,Und,-H,0,redEnd ^{1,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_4)--??1S$MONO,Und,-H,0,redEnd
374.73 0.0
375.28 0.0
375.8 0.0
376.36 0.0
376.88 0.0
377.44 0.0
377.95 0.0
383.3 2.3
383.86 0.0

Annotations:

redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
384.38 0.0

Annotations:

redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--??1S/#lcleavage$MONO,Und,-H,0,redEnd
384.96 1.7
385.52 0.0
386.03 0.0
386.59 0.0
387.11 0.0
387.65 0.0
388.19 0.0
388.72 0.0
389.28 0.0
389.8 0.0

Annotations:

^{3,5}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_3_5)--??1S$MONO,Und,-H,0,redEnd
391.24 0.5
391.79 0.0
392.35 0.0
392.87 0.0
393.44 0.0
393.95 0.0
394.5 0.0
395.03 0.0
395.57 0.0
395.98 0.5
396.71 0.5
397.27 0.0
397.79 0.0
398.14 0.0
398.36 0.0
398.87 0.0
399.21 0.0
399.44 0.0
399.95 0.0
400.51 0.0
401.03 0.0
402.16 0.8
402.79 5.7
403.36 0.0
403.94 2.8

Annotations:

^{0,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_4)--??1S$MONO,Und,-H,0,redEnd ^{1,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_3)--??1S$MONO,Und,-H,0,redEnd ^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--??1S$MONO,Und,-H,0,redEnd
404.31 1.4

Annotations:

^{0,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_4)--??1S$MONO,Und,-H,0,redEnd ^{1,3}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_1_3)--??1S$MONO,Und,-H,0,redEnd ^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--??1S$MONO,Und,-H,0,redEnd
404.87 0.0
405.17 0.0
405.44 0.0
405.95 0.0
406.24 0.0
406.52 0.0
407.03 0.0
407.57 0.0
408.11 0.0
408.64 0.0
409.19 0.0
415.86 0.7
416.44 0.0
416.95 0.0
417.51 0.0
418.03 0.0
418.57 0.0
419.06 3.4
419.6 1.3
419.97 1.6
420.95 5.9
421.26 1.0
421.6 0.0
422.03 6.4
422.67 0.0
423.01 4.9
423.56 0.0
424.11 0.0
425.1 18.5
425.88 0.8
426.09 0.7
426.89 2.3
427.49 0.0
428.04 0.0
428.87 1.4
429.44 0.0
429.96 0.0
430.5 2.8
431.05 0.0
431.61 0.0
432.32 2.9
432.94 2.3
433.62 0.0
434.16 0.0
434.7 0.0
435.23 0.0
435.78 0.0
436.3 0.0
436.86 0.0
437.38 0.0

There are 6 similar spectra:

Id Structure Score

7678

0.82

7685

0.82

7161

0.80

7697

0.71

4830

0.62

6648

0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 463.12
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

Report errors in this entry

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