Structure

    redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
137.95 0.2
151.07 0.1
156.08 2.1
156.65 0.0
172.86 0.3
198.1 0.3
201.23 0.1
202.06 0.3
204.05 1.7

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
204.23 1.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
205.06 0.3

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
212.06 0.2
212.94 0.7
217.82 0.2
222.13 0.2

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
223.04 0.3

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
223.29 0.5
223.97 0.9
224.84 0.2
229.0 0.5
234.12 0.4
234.9 0.9
235.45 0.0
240.11 0.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
241.01 12.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
242.07 1.9
242.32 0.8
242.85 0.0
257.96 0.3
259.07 2.5

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
259.49 0.5

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
260.14 0.4
261.13 0.3
265.73 0.2
279.2 0.1
281.24 0.2
283.31 1.1
283.73 0.4
284.09 2.3
284.69 0.0
285.16 1.2
285.75 0.0
302.08 18.0
303.1 1.5
303.67 0.0
304.01 0.3
306.5 0.3
314.17 1.0
314.73 0.0
326.36 0.5
327.08 0.3
330.87 0.2
333.12 0.1
343.13 1.2
344.13 6.1
345.03 1.2
346.16 0.5
355.63 0.3
356.13 0.2
357.17 0.6
357.71 0.0
359.07 0.4
360.02 0.1
361.12 0.4
363.99 0.2
365.27 0.5
367.31 0.6
373.05 0.2
373.33 0.5
374.2 0.7
374.77 0.0
384.0 0.2

Annotations:

redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S/#lcleavage$MONO,Und,-H,0,redEnd
385.22 0.1
392.99 1.1
393.61 0.2
400.22 0.2
402.03 0.6
403.14 1.2
404.08 0.6
404.37 0.4
405.26 0.4
416.37 0.5
418.04 0.1
419.11 0.9
419.67 0.0
421.12 0.6
421.67 0.0
422.32 0.5
429.21 0.1
431.31 0.5
432.91 0.4
433.1 0.7
433.67 0.0
436.3 0.4
463.99 1.3

Annotations:

redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
464.32 0.8

Annotations:

redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
464.8 0.3

Annotations:

redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
465.11 0.8
465.39 0.8
465.97 0.0

There are 37 similar spectra:

Id Structure Score

5386

0.80

0.80

7678

0.79

7685

0.79

0.79

4830

0.75

0.75

5537

0.70

7697

0.67

0.67

6648

0.65

0.65

0.64

6504

0.61

6512

0.61

6520

0.61

6578

0.61

6442

0.60

6503

0.60

6511

0.60

6519

0.60

6577

0.60

0.60

7397

0.59

7426

0.59

3489

0.59

7679

0.58

7686

0.58

0.58

7971

0.57

6649

0.56

0.56

6690

0.55

0.55

5410

0.53

6696

0.53

3490

0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 463.12
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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