Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p)--??1S)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

The O-glycome of human nigrostriatal tissue and its alteration in Parkinsons disease

GlyTouCan Accession Number: Loading...

Peak Intensity
290.24 1.3

Annotations:

B ?1D-Gal,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
305.85 0.4
314.88 1.0
322.98 0.6
326.1 0.9
370.1 17.5
370.32 9.1
371.33 2.3
379.18 1.0
408.04 0.5
435.05 1.2
446.05 1.4

Annotations:

ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p/#zcleavage)--??1S)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p/#ycleavage)--??1S)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
462.07 2.3
463.42 0.9
464.2 431.6

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p/#ycleavage)--??1S)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
465.33 3.2
478.31 0.8
491.97 0.5
493.15 0.9
503.32 1.2
527.45 1.2
534.06 0.5
559.14 0.6
564.52 0.7
578.3 0.6
590.45 1.0
607.46 1.3
617.35 1.5
641.37 1.1
655.08 0.6
658.27 0.4
660.24 0.4
673.44 2.1
675.33 217.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p/#ycleavage)--??1S/#lcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p)--??1S/#lcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
676.44 0.6
677.74 0.8
683.42 0.5
684.26 0.7
695.19 2.7
709.47 0.6
710.2 0.7
711.24 43.9
711.51 23.9
712.06 3.5
712.46 2.3
713.48 1.6
714.74 0.5
718.24 1.4
731.19 0.4
737.28 2.1

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p/#zcleavage)--??1S)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p)--??1S)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
738.49 1.0
739.31 1.2
747.36 1.1
753.48 1.5
754.34 5.4
755.29 201.7

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p/#ycleavage)--??1S)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p)--??1S)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
756.1 0.4
782.22 1.0
785.3 1.8
786.87 1.0
788.75 0.6
806.95 1.1
814.28 1.0
825.15 0.4
829.28 0.5
837.25 0.7
840.73 2.0
848.68 0.8
871.84 0.5
873.89 1.3
885.13 1.0
892.43 2.6
892.73 8.4
893.45 1.0
895.12 1.6
900.38 1.4
913.74 0.9
917.84 1.3
922.24 0.6
922.55 2.0
930.93 1.1
932.41 0.6
948.18 1.0
955.65 0.8
965.21 3.3
966.37 41.1

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p)--??1S/#lcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
968.34 2.1
973.8 1.9
983.42 1.8
985.42 1.0
986.09 1.6
988.55 0.5
1002.37 4.5
1002.67 1.0
1005.89 3.0
1006.48 0.7
1008.27 1.4
1010.36 2.7
1016.11 1.6
1024.98 1.6

There are 5 similar spectra:

Id Structure Score

1.00

6595

0.93

0.93

4879

0.88

0.88

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1045.31
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Homo Sapiens
Tissue - Brain

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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