Structure

    redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
203.93 3.2

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
205.04 1.5

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
205.4 1.3
212.13 1.4
217.98 1.7
222.08 13.1

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
223.1 3.2
230.1 5.0
231.25 1.9
235.55 1.9
236.1 3.2
238.03 3.0
244.21 1.5
257.05 1.7
263.74 1.9
276.09 2.2
283.79 3.9
288.25 1.8
289.01 1.4
290.02 2.0
292.18 3.5
304.27 2.2
306.12 262.2
307.15 23.7
308.18 8.6
309.98 1.7
324.21 10.5
325.24 1.2
347.08 1.7

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
348.3 7.5

Annotations:

B ?1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
365.15 1.6

Annotations:

C ?1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.03 2.4

Annotations:

C ?1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
371.21 1.5
384.19 427.3

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
385.35 32.8
386.01 1.2
389.52 1.5
405.26 5.1
407.41 8.3
408.36 1.7
409.15 2.1
425.26 42.7
426.14 7.3
461.31 11.2
461.78 1.3
467.13 8.7
467.66 2.4
468.15 2.7
475.13 1.3
477.29 1.4
477.5 1.5
478.17 1.7
479.5 12.5
479.74 3.2
480.23 9.1
484.93 2.0
493.2 4.9
493.46 3.4
494.31 1.8
495.2 10.6
497.03 1.3
501.96 1.3
509.17 6.2
511.32 2.6
513.38 15.4
515.16 1.2
518.4 1.9
521.27 131.1
522.26 24.2
524.13 2.6
529.28 1.7
531.36 3.2
548.15 1.6
550.13 1.3
551.05 3.0
551.38 2.3
569.28 104.5

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
570.18 19.2

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
578.54 1.5
584.37 1.3
587.38 5.6

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.44 6.8
589.08 1.6
589.37 1.8
620.97 1.7
624.02 3.1
625.09 3.0
638.36 152.1
639.34 30.0
640.12 1.4
640.45 6.4
655.16 2.0
656.41 5.5
657.41 1.2
673.38 1.3
688.41 1.3
688.97 2.1
696.21 3.9
696.42 2.8
697.56 2.5

There are 11 similar spectra:

Id Structure Score

5426

0.88

5427

0.83

7167

0.82

4804

0.82

0.82

6802

0.82

7168

0.82

5709

0.82

5710

0.82

4847

0.81

0.81

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 732.30
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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