Structure

    redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
344.69 0.6
347.03 0.3

Annotations:

BZ ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
362.99 1.1
363.54 0.0

Annotations:

BY ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
387.3 0.5

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
403.77 0.3
404.71 2.7

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
405.26 6.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
406.11 0.6

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
407.34 0.6

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
447.49 1.2
510.48 0.7

Annotations:

B ?1HexNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
527.17 0.6
528.56 0.3

Annotations:

C ?1HexNAc,p/#ccleavage--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
550.47 2.7

Annotations:

ZZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.02 0.8

Annotations:

ZZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
567.17 0.6

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
567.49 0.7

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
568.21 0.6
568.97 0.3

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
572.01 1.1
572.55 0.0
587.44 2.9

Annotations:

YY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.3 1.2
610.16 1.4

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
610.7 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
611.36 0.4
628.12 2.5

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
628.57 0.5

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
629.39 1.1
640.57 1.9
641.12 0.0
670.09 2.3
670.43 0.6
671.66 2.0
672.21 0.0
681.05 0.3
682.49 3.8
684.87 0.3
688.33 1.0
712.59 2.7

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
713.13 0.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
722.81 0.4
729.53 1.0
730.35 42.3

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
731.48 13.5

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
732.06 0.0

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
736.49 1.0
748.14 1.6
748.61 1.2
752.52 2.0

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
753.08 0.0

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
768.96 0.9
772.47 2.7

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
773.04 0.0

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
779.25 0.5
788.45 0.6

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
790.38 75.7

Annotations:

YY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
791.38 13.9
791.68 4.3
792.22 0.0
799.7 1.4
800.25 0.0
826.34 0.8
867.43 6.4
867.77 5.6
868.42 8.4
869.01 0.0
869.54 0.2
875.32 0.4
878.63 0.1
885.04 1.3
885.5 1.6
886.19 0.6
886.46 0.4
889.33 1.2
889.87 0.0
894.75 0.9
898.48 0.9
899.41 1.8
899.95 0.0
915.5 0.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
916.49 4.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
917.21 0.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
917.43 1.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
918.15 1.2

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
918.7 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
919.55 1.7
920.11 0.0
927.36 25.0
927.85 4.1
928.37 4.6
928.62 4.0
929.24 0.0
936.49 4.4

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
937.07 0.0

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
937.55 2.2
938.1 0.0
939.74 1.9
940.29 0.0

There are 8 similar spectra:

Id Structure Score

4890

0.94

0.94

7176

0.93

4816

0.92

0.92

5460

0.88

5457

0.88

5456

0.81

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1138.45
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

Report errors in this entry

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