Structure

    redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
364.21 3.8

Annotations:

BY a1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ a1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.33 1.8
369.26 2.7
373.27 1.8
386.76 5.2
387.18 16.0

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
389.11 2.1

Annotations:

ZZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd
405.14 42.1

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
407.14 45.7

Annotations:

YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd
407.5 5.2
425.24 13.3

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd
437.36 3.3
459.08 3.0
478.33 2.4
482.0 2.3
510.23 2.3

Annotations:

B a1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
515.45 3.5
527.37 10.0

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
527.71 2.4

Annotations:

C a1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
545.11 1.9
549.91 6.0
550.37 5.7
551.29 9.4

Annotations:

ZZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd
551.48 4.6
567.21 10.4

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
567.43 7.7
568.34 8.7

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
569.23 10.4

Annotations:

YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd
587.25 128.3

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd
610.34 3.9

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
628.28 14.9

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
640.2 1.7
661.94 3.5
664.35 1.8
670.33 2.9
682.28 13.7
687.81 1.7
688.27 5.5
694.08 6.5
694.36 2.4
704.37 4.7
712.06 6.1

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
712.39 2.9
724.22 7.4
730.31 309.3

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
748.32 8.3
748.65 3.9
751.93 2.1

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
752.99 2.1
753.25 5.4
754.56 4.7

Annotations:

ZZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd
772.22 29.6

Annotations:

YZ redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
772.64 5.4
776.99 6.2
777.17 4.6
790.29 567.5

Annotations:

YY redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
791.21 3.5
804.22 3.7
807.35 3.0
825.05 2.2
825.36 8.3
828.17 3.9
845.72 1.8
849.53 2.2
867.27 93.8
867.77 5.9
871.17 2.4
885.32 82.8
888.42 3.0

Annotations:

^{1,5}A_{GalNAc} a1D-GalNAc,p/#acleavage_1_5(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
897.4 2.5
898.43 2.3
900.4 2.3
914.98 3.4
915.52 3.8
916.42 6.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
916.68 3.4
918.29 16.4

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd
927.42 133.2
929.39 3.8
933.13 2.3
936.3 6.6

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd
936.52 4.6
938.36 1.9
939.03 1.8
957.2 8.7
975.43 42.3

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
993.28 2.4

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
994.44 8.3
1019.09 1.9
1031.27 7.0
1031.55 5.2
1037.32 7.3
1037.59 5.1
1060.33 1.8
1061.24 2.1
1078.44 2.3
1079.4 240.3
1080.59 2.5
1097.31 13.1
1097.6 5.1

There are 7 similar spectra:

Id Structure Score

4890

0.96

7176

0.92

5460

0.89

4816

0.89

7155

0.88

5456

0.88

5458

0.65

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1138.45
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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